{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T06:43:03Z","timestamp":1777444983317,"version":"3.51.4"},"reference-count":14,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2024,10,15]],"date-time":"2024-10-15T00:00:00Z","timestamp":1728950400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:p>\n                    An elderly patient who presented with 1\u00a0week of fever and respiratory symptoms was tested for dengue infection with an Abbott Bioline\u2122 Dengue Duo immunochromatographic assay. Unexpectedly, the control line of the dengue non-structural protein 1 (NS1) component was absent, necessitating result invalidation. It remained absent when the test was repeated on the SD Biosensor Standard\u2122 Q Dengue Duo, but present on the Wells Bio careUS\n                    <jats:sup>TM<\/jats:sup>\n                    Dengue Combo and Asan Easy Test\u00ae Dengue DUO assays, suggesting potential interference. Dilution, polyethylene glycol (PEG) precipitation and centrifugation with a 100\u00a0kDa filter were performed to reduce\/remove the potential interferent. Sera from other patients that showed a control line, and a test line that was either positive or negative for NS1, were used as controls. Upon dilution with negative control serum, a faint control line emerged. PEG precipitation resulted in disappearance of control and test lines in the positive control. Filtration led to emergence of the control line for the patient\u2019s serum but caused the test line for the positive control serum to disappear. Overall, investigations suggested the presence of a high molecular weight (&gt;100\u00a0kDa) substance which interferes with chicken IgY-anti-chicken IgY binding at the control line of affected assays. Our results highlight two important points: firstly, some commonly used laboratory procedures (e.g. PEG or filtration) may inadvertently remove the target biomarker (e.g. multimeric NS1) and should be interpreted with appropriate controls. Secondly, alternative kits that use a different antigen-antibody combination for the control line can be considered when similar patients are encountered in future.\n                  <\/jats:p>","DOI":"10.1177\/00045632241292430","type":"journal-article","created":{"date-parts":[[2024,10,16]],"date-time":"2024-10-16T01:15:01Z","timestamp":1729041301000},"page":"148-152","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["A curious case of a negative control line on a dengue duo assay due to interference \u2013 A case report"],"prefix":"10.1177","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2497-7046","authenticated-orcid":false,"given":"Sheila X.","family":"Soh","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, National University Hospital, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4272-0001","authenticated-orcid":false,"given":"Tze Ping","family":"Loh","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, National University Hospital, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sharon","family":"Saw","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, National University Hospital, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ai Teng","family":"Chong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, National University Hospital, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia Sing","family":"Yap","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, National University Hospital, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunil K.","family":"Sethi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, National University Hospital, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1499-3640","authenticated-orcid":false,"given":"Lizhen","family":"Ong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, National University Hospital, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2024,10,15]]},"reference":[{"issue":"8","key":"e_1_3_3_2_2","first-page":"180","article-title":"Dengue incidence trends and its burden in major endemic regions from 1990 to 2019","volume":"7","author":"Tian N","year":"2022","unstructured":"Tian N, Zheng JX, Guo ZY, et al. Dengue incidence trends and its burden in major endemic regions from 1990 to 2019. Trav Med Infect Dis 2022; 7(8): 180.","journal-title":"Trav Med Infect Dis"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05285-11"},{"key":"e_1_3_3_4_2","volume-title":"Bioline\u2122 dengue duo","year":"2020","unstructured":"Bioline\u2122 dengue duo [package insert]. Gyeonggi-do, Republic of Korea: Abbott Diagnostics Korea Inc.; 2020."},{"key":"e_1_3_3_5_2","volume-title":"Standard\u2122 Q dengue duo","year":"2022","unstructured":"Standard\u2122 Q dengue duo [package insert]. Gyeonggi-do, Republic of Korea: SD Biosensor, Inc.; 2022."},{"key":"e_1_3_3_6_2","volume-title":"careUS\u2122 dengue Combo NS1 & IgM\/IgG","year":"2019","unstructured":"careUS\u2122 dengue Combo NS1 & IgM\/IgG [package insert]. Gyeonggi-do, Republic of Korea: Wells Bio, Inc.; 2019."},{"key":"e_1_3_3_7_2","unstructured":"Asan Easy Test\u00ae dengue DUO [package insert]. Gyeonggi-do Republic of Korea: Asan Pharmaceutical Co. Ltd.. Year not stated."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2019-0898"},{"issue":"2","key":"e_1_3_3_9_2","first-page":"105","article-title":"Interferences in immunoassay","volume":"25","author":"Tate J","year":"2004","unstructured":"Tate J, Ward G. Interferences in immunoassay. Clin Biochem Rev 2004; 25(2): 105\u2013120.","journal-title":"Clin Biochem Rev"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2021-1288"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01415"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34415-1"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20150012"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/45.5.616"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/45.7.942"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00045632241292430","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/00045632241292430","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00045632241292430","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:25:31Z","timestamp":1777379131000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00045632241292430"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,15]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1177\/00045632241292430"],"URL":"https:\/\/doi.org\/10.1177\/00045632241292430","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,15]]}}}