{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:56:01Z","timestamp":1791845761125,"version":"4.3.4"},"reference-count":9,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Measurement of cerebrospinal fluid (CSF) kappa free light chains (KFLCs) for the detection of intrathecal immunoglobulin synthesis has generated interest as an alternative to CSF oligoclonal bands due to its rapid turnaround time and ability to automate the assay. A turbidimetric immunoassay \u2013 Freelite Mx, is available from the Binding Site, but published data on performance is scant. Therefore, we undertook a multicentre sample comparison and investigated reagent lot-to-lot-variation (LTLV).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Intra-\/inter-assay accuracy and imprecision of the Freelite Mx assay on the Binding Site Optilite analyser was assessed. Twenty paired CSF\/serum samples were sent to three laboratories within Australia for the measurement of CSF\/serum KFLC\/albumin and concentrations compared using the Kruskal\u2013Wallis test, Spearman\u2019s rank (rs), and Passing-Bablok analysis. Lot-to-lot-variation between three reagent lots was undertaken by analysis of 20 CSF samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Intra- and inter-assay imprecision was \u22644.4 and \u22644.1%, respectively. There was a good correlation (rs = \u22650.98) between sites for the measured CSF KFLC concentration, and no significant difference in the median concentration measured between sites (3.31, 2.78, and 3.48\u00a0mg\/L,\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      = .98). The median bias between reagent lots was &lt;4%, the intercept of the regression between lots was between \u22120.02 and 0.06\u00a0mg\/L, and the slope ranged from 0.96 to 1.07.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Overall, there was a good agreement in CSF KFLC concentrations among laboratories, and LTLV was deemed acceptable. Ascertaining biological variability of CSF KFLCs and the participation of laboratories in quality assurance schemes would assist with harmonisation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00045632251337616","type":"journal-article","created":{"date-parts":[[2025,4,12]],"date-time":"2025-04-12T17:13:34Z","timestamp":1744478014000},"page":"505-509","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Performance of the Binding Site cerebrospinal fluid kappa free light chains assay"],"prefix":"10.1177","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9237-8136","authenticated-orcid":false,"given":"Simon Alfred","family":"Handley","sequence":"first","affiliation":[{"name":"Royal Hobart Hospital"},{"name":"University of Tasmania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Barnes","sequence":"additional","affiliation":[{"name":"Pathwest QE II MC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Alfred Pathology Service"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4703-0488","authenticated-orcid":false,"given":"Troy","family":"Wanandy","sequence":"additional","affiliation":[{"name":"University of Tasmania"},{"name":"Royal Hobart Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,4,29]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/1352458519845844"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/13524585221134213"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/13524585221134217"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2017.03.012"},{"key":"e_1_3_4_6_2","first-page":"51","article-title":"Lot-to-Lot variation","volume":"39","author":"Thompson S","year":"2018","unstructured":"Thompson S, Chesher D. Lot-to-Lot variation. Clin Biochem Rev 2018; 39: 51\u201360.","journal-title":"Clin Biochem Rev"},{"key":"e_1_3_4_7_2","volume-title":"The EFLM biological variation database","author":"Aarsand AK","unstructured":"Aarsand AK, Fernandez-Calle P, Webster C, et al. The EFLM biological variation database. https:\/\/biologicalvariation.eu\/ (accessed 26 September 2024)."},{"key":"e_1_3_4_8_2","volume-title":"Cerebrospinal fluid analysis 08 \u2013 free light chains Kappa","author":"Instand","unstructured":"Instand. Cerebrospinal fluid analysis 08 \u2013 free light chains Kappa. https:\/\/www.instand-ev.de\/en\/ (accessed 26 September 2024)."},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom12050677"},{"key":"e_1_3_4_10_2","first-page":"355","article-title":"Overestimation of albumin measured by bromocresol green vs bromocresol purple method: influence of acute-phase globulins","volume":"49","author":"Garcia Moreira V","year":"2018","unstructured":"Garcia Moreira V, Beridze Vaktangova N, Martinez Gago MD, et al. Overestimation of albumin measured by bromocresol green vs bromocresol purple method: influence of acute-phase globulins. Lab Med 2018; 49: 355\u2013361.","journal-title":"Lab Med"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00045632251337616","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/00045632251337616","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00045632251337616","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T12:25:40Z","timestamp":1777379140000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00045632251337616"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,29]]},"references-count":9,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,11]]}},"alternative-id":["10.1177\/00045632251337616"],"URL":"https:\/\/doi.org\/10.1177\/00045632251337616","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,29]]}}}