{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T08:03:44Z","timestamp":1780041824244,"version":"3.53.1"},"reference-count":6,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["Ann Clin Biochem"],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The significance level specified for a statistical analysis indicates the expected false positive rate. Failure to confine false positives to their expected rates can lead to misleading conclusions and especially in studies investigating required sample sizes. A simulation study was used to investigate false positives associated with regression analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Randomly generated X, Y pairs with zero underlying bias were subjected to Deming regression analysis. The design incorporated equal X, Y errors, unequal but parallel errors and non-parallel errors, each in combination with small (2:1), moderate (10:1) and large (2667:1) maximum:minimum range ratios. Bias (false positives in this context) was signified by failure of 95% confidence intervals (CIs) or, alternatively, the joint 95% slope, intercept confidence region (CR) to enclose target values.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>False positive rates assessed by CIs ranged from 6 to 10% and were clearly range ratio dependent, while those assessed by CRs were stable and very close to the expected 5% throughout.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CRs are distinctly more reliable than CIs in controlling false positive rates and should be the preferred option when using regression analysis in methods and reagent-lot comparisons. The computer program used to perform the study is freely available.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1177\/00045632261454285","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T02:11:46Z","timestamp":1778638306000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Methods and reagent-lot comparisons by regression analysis: False positives"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1239-2905","authenticated-orcid":false,"given":"William A","family":"Sadler","sequence":"first","affiliation":[{"name":"Christchurch Hospital"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,5,12]]},"reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00769-010-0674-9"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/00045632241252006"},{"key":"e_1_3_4_4_2","unstructured":"Sadler WA. Regression accuracy and power simulations. https:\/\/www.aacb.asn.au\/AACB\/Resources\/Regression-accuracy-and-power-simulations.aspx 2011 accessed 24 October 2024."},{"key":"e_1_3_4_5_2","volume-title":"Statistical adjustment of data","author":"Deming WE","year":"1943","unstructured":"Deming WE. Statistical adjustment of data. New York: John Wiley & Sons, 1943."},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2022-0274"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)90837-8"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00045632261454285","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/00045632261454285","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00045632261454285","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T07:23:11Z","timestamp":1780039391000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00045632261454285"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,12]]},"references-count":6,"alternative-id":["10.1177\/00045632261454285"],"URL":"https:\/\/doi.org\/10.1177\/00045632261454285","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,12]]},"article-number":"00045632261454285"}}