{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:01:29Z","timestamp":1777424489150,"version":"3.51.4"},"reference-count":15,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[1982,3,1]],"date-time":"1982-03-01T00:00:00Z","timestamp":383788800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[1982,3]]},"abstract":"<jats:p>\n                    A simple method which uses 100 \u03bcl serum has been developed to measure the binding of somatomedin B (SMB) to protein in serum from normal children, adults, and patients with growth hormone deficiency.\n                    <jats:sup>125<\/jats:sup>\n                    I-labelled SMB was used as binding ligand. The correlation between the binding of label by the proposed procedure and by an immunoelectrophoretic technique was acceptable (r=0\u00b773; p &lt;0\u00b702). The mean percentage of label bound to protein in serum from patients with deficiencies of growth hormone or other trophic hormones was significantly (p&lt;0\u00b7001) lower than that for controls. The physicochemical characteristics of a specific binding-protein suggested that a protein with low capacity (160 pmol\/l) and low K\n                    <jats:sub>a<\/jats:sub>\n                    (4\u00b737 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    l\/mol was present in serum, in addition to a high concentration of alpha globulin(s) which also bound\n                    <jats:sup>125<\/jats:sup>\n                    I SMB.\n                  <\/jats:p>","DOI":"10.1177\/000456328201900204","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:57:08Z","timestamp":1391619428000},"page":"83-88","source":"Crossref","is-referenced-by-count":0,"title":["A Screening Method for Measuring Somatomedin B Protein Binding in Serum from Children with Growth Retardation"],"prefix":"10.1177","volume":"19","author":[{"given":"B T","family":"Rudd","sequence":"first","affiliation":[{"name":"Department of Clinical Endocrinology, Birmingham & Midland Hospital for Women, Sparkhill, Birmingham B11 4HL"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P H W","family":"Rayner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan M","family":"Stafford","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Halliday","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1982,3,1]]},"reference":[{"key":"bibr1-000456328201900204","first-page":"259","volume":"30","author":"Van Wyck JJ","year":"1974","journal-title":"Recent Prog Harm Res"},{"key":"bibr2-000456328201900204","first-page":"1","volume":"163","author":"Hall K","year":"1972","journal-title":"Acta Endocrinol (Copenh)"},{"key":"bibr3-000456328201900204","unstructured":"Van Wyk JJ, Underwood LE, Hintz RL,  Chemical properties and some biological effects of human somatomedin. In: Raiti S, ed. Advances in human growth hormone research, 1973. USA (DHEW Publication No. (NIH)74\u2013612, 25\u201349)."},{"key":"bibr4-000456328201900204","first-page":"98","author":"Van den Brande JL","journal-title":"Advances in human growth hormone research"},{"key":"bibr5-000456328201900204","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-027308-9.50009-3"},{"key":"bibr6-000456328201900204","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(75)90296-9"},{"key":"bibr7-000456328201900204","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-45-5-988"},{"key":"bibr8-000456328201900204","doi-asserted-by":"publisher","DOI":"10.1210\/endo-104-4-1107"},{"key":"bibr9-000456328201900204","doi-asserted-by":"publisher","DOI":"10.1210\/endo-104-4-1143"},{"key":"bibr10-000456328201900204","first-page":"1","volume-title":"Handbook of experimental immunology","volume":"16","author":"Pinckard RN","year":"1973"},{"key":"bibr11-000456328201900204","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2795(71)90123-1"},{"key":"bibr12-000456328201900204","first-page":"349","volume-title":"Somatomedins and growth","author":"Rudd BT","year":"1979"},{"key":"bibr13-000456328201900204","doi-asserted-by":"crossref","first-page":"131p","DOI":"10.1677\/joe.0.0810131","volume":"81","author":"Salacinski P","year":"1979","journal-title":"J Endocrinol"},{"key":"bibr14-000456328201900204","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107903"},{"key":"bibr15-000456328201900204","first-page":"7","volume-title":"Somatomedins and growth","author":"Fryklund L","year":"1979"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456328201900204","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456328201900204","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:26:56Z","timestamp":1777375616000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/000456328201900204"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,3]]},"references-count":15,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1982,3]]}},"alternative-id":["10.1177\/000456328201900204"],"URL":"https:\/\/doi.org\/10.1177\/000456328201900204","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,3]]}}}