{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:01:57Z","timestamp":1777424517637,"version":"3.51.4"},"reference-count":17,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[1982,3,1]],"date-time":"1982-03-01T00:00:00Z","timestamp":383788800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[1982,3]]},"abstract":"<jats:p>\n                    A new method, employing the mild oxidant n-bromosuccinimide, for the iodination of proteins for radioimmunoassay is described. Human prolactin, follicle-stimulating, luteinising, thyroid-stimulating, and growth hormones were iodinated with\n                    <jats:sup>125<\/jats:sup>\n                    I by this method to specific activities up to 136 \u03bcCi\/\u03bcg. Incorporation of iodine ranged from 67% to 83% using 2\u03bcg hormone, 200 \u03bcCi Na\n                    <jats:sup>125<\/jats:sup>\n                    I, 0\u00b75 nmol oxidant, and a reaction time of 15s. In comparative studies, the immunological activity after iodination by this method was equal to, or greater than, that by the chlorine gas, Iodo-Gen, or chloramine-T methods. The reagent is cheap and stable, and the method permits efficient use of antigen and iodide.\n                  <\/jats:p>","DOI":"10.1177\/000456328201900214","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:57:08Z","timestamp":1391619428000},"page":"129-133","source":"Crossref","is-referenced-by-count":39,"title":["Use of n-Bromosuccinimide for the Iodination of Proteins for Radioimmunoassay"],"prefix":"10.1177","volume":"19","author":[{"given":"Paul","family":"Reay","sequence":"first","affiliation":[{"name":"Department of Clinical Endocrinology, Birmingham and Midland Hospital for Women, Birmingham B11 4HL"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1982,3,1]]},"reference":[{"key":"bibr1-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1038\/194495a0"},{"key":"bibr2-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0600527"},{"key":"bibr3-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0550453"},{"key":"bibr4-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1038\/182053a0"},{"key":"bibr5-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(78)91322-0"},{"key":"bibr6-000456328201900214","doi-asserted-by":"crossref","first-page":"131P","DOI":"10.1677\/joe.0.0810131","volume":"81","author":"Salacinski P","year":"1979","journal-title":"J Endocrinol"},{"key":"bibr7-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1042\/bj1130299"},{"key":"bibr8-000456328201900214","first-page":"64","volume":"13","author":"Pennisi F","year":"1969","journal-title":"J Nucl Biol Med"},{"key":"bibr9-000456328201900214","volume-title":"Radioiodination techniques","author":"Bolton AE","year":"1977"},{"key":"bibr10-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-34-1-23"},{"key":"bibr11-000456328201900214","first-page":"XXX","volume":"55","author":"Bolton AE","year":"1972","journal-title":"J Endocrinol"},{"key":"bibr12-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-33-5-732"},{"key":"bibr13-000456328201900214","first-page":"269","volume-title":"Radioimmunoassay in clinical biochemistry","author":"Martin MJ","year":"1975"},{"key":"bibr14-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1210\/endo-95-5-1228"},{"key":"bibr15-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.0580275"},{"key":"bibr16-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/25.10.1826"},{"key":"bibr17-000456328201900214","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(75)90266-3"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456328201900214","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456328201900214","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:26:58Z","timestamp":1777375618000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/000456328201900214"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,3]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1982,3]]}},"alternative-id":["10.1177\/000456328201900214"],"URL":"https:\/\/doi.org\/10.1177\/000456328201900214","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[1982,3]]}}}