{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:08:01Z","timestamp":1777424881293,"version":"3.51.4"},"reference-count":8,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[1983,3,1]],"date-time":"1983-03-01T00:00:00Z","timestamp":415324800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[1983,3]]},"abstract":"<jats:p>\n                    A double antibody, semi-automated radioimmunoassay for serum prostatic acid phosphatase (PAP) is described, which uses the\n                    <jats:sup>125<\/jats:sup>\n                    I-labelled N-succinimidyl-3-(4-hydroxyphenyl) propionate ester of PAP. This type of label has substantially higher immunoreactivity than that prepared using the chloramine-T method of radioiodination. Chromatographic purification of either label on Ultrogel ACA44 further improved immunoreactivity. The lowest detection limit (0\u00b735 \u03bcg\/l) was achieved with the chromatographically purified ester label.\n                  <\/jats:p>","DOI":"10.1177\/000456328302000210","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:57:08Z","timestamp":1391619428000},"page":"112-115","source":"Crossref","is-referenced-by-count":2,"title":["Comparison of Bolton-Hunter and Chloramine-T Techniques for the Radioiodination of Prostatic Acid Phosphatase"],"prefix":"10.1177","volume":"20","author":[{"given":"C M","family":"Sturgeon","sequence":"first","affiliation":[{"name":"Department of Clinical Chemistry, University of Edinburgh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Beynon","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Edinburgh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Seth","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry, University of Edinburgh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G D","family":"Chisholm","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Edinburgh"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1983,3,1]]},"reference":[{"key":"bibr1-000456328302000210","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197712222972501"},{"key":"bibr2-000456328302000210","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(81)90103-0"},{"key":"bibr3-000456328302000210","doi-asserted-by":"publisher","DOI":"10.3109\/01485017808988340"},{"key":"bibr4-000456328302000210","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/24.3.466"},{"key":"bibr5-000456328302000210","first-page":"267","volume-title":"Immunoassays for clinical chemistry","author":"Brown NS","year":"1983","edition":"2"},{"key":"bibr6-000456328302000210","doi-asserted-by":"publisher","DOI":"10.1042\/bj1330529"},{"key":"bibr7-000456328302000210","first-page":"573","volume":"24","author":"Bagshawe KD.","year":"1975","journal-title":"Laboratory Practice"},{"key":"bibr8-000456328302000210","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/26.13.1854"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456328302000210","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456328302000210","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:28:01Z","timestamp":1777375681000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/000456328302000210"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1983,3]]},"references-count":8,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1983,3]]}},"alternative-id":["10.1177\/000456328302000210"],"URL":"https:\/\/doi.org\/10.1177\/000456328302000210","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[1983,3]]}}}