{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:10:53Z","timestamp":1777425053236,"version":"3.51.4"},"reference-count":19,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[1984,3,1]],"date-time":"1984-03-01T00:00:00Z","timestamp":446947200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[1984,3]]},"abstract":"<jats:p>Purine and pyrimidine nucleosides and bases were analysed using an isocratic reverse-phase HPLC technique and utilising simple sample preparation. The method has been successfully used to separate a large number of biologically occurring nucleic acid products within a 30-minute period and to identify them by their chromatographic and optical properties. A number of metabolic disorders have been identified, including hypoxanthine-guanine phosphoribosyl transferase and ornithine carbamyl transferase deficiencies. The method has been in routine use for 2 years performing both quantitative and qualitative analysis and has proved to be robust and reliable.<\/jats:p>","DOI":"10.1177\/000456328402100210","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:57:08Z","timestamp":1391619428000},"page":"131-136","source":"Crossref","is-referenced-by-count":14,"title":["Experience with a Simple High-Performance Liquid Chromatography Method for the Analysis of Purine and Pyrimidine Nucleosides and Bases in Biological Fluids"],"prefix":"10.1177","volume":"21","author":[{"given":"M J","family":"Bennett","sequence":"first","affiliation":[{"name":"Department of Chemical Pathology, The Children's Hospital, Western Bank, Sheffield S10 2TH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K H","family":"Carpenter","sequence":"additional","affiliation":[{"name":"Department of Chemical Pathology, The Children's Hospital, Western Bank, Sheffield S10 2TH, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1984,3,1]]},"reference":[{"key":"bibr1-000456328402100210","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)84111-X"},{"key":"bibr2-000456328402100210","first-page":"282","volume":"14","author":"Brown PR","year":"1976","journal-title":"J Clin Chem Clin Biochem"},{"key":"bibr3-000456328402100210","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)88205-9"},{"key":"bibr4-000456328402100210","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)95285-3"},{"key":"bibr5-000456328402100210","first-page":"53","volume-title":"Current Developments in the Clinical applications of HPLC, G.C. and M.S","author":"Brown PR","year":"1980"},{"key":"bibr6-000456328402100210","first-page":"295","volume":"10","author":"Brown PR","year":"1979","journal-title":"Chromtogr Sci"},{"key":"bibr7-000456328402100210","doi-asserted-by":"publisher","DOI":"10.1007\/BF02263578"},{"key":"bibr8-000456328402100210","unstructured":"Stanbury JB, Wyngarden JB, Frederickson DS, The Metabolic Basis of Inherited Disease, 5th edn. 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