{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:15:38Z","timestamp":1777425338611,"version":"3.51.4"},"reference-count":11,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[1985,11,1]],"date-time":"1985-11-01T00:00:00Z","timestamp":499651200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[1985,11]]},"abstract":"<jats:p>The LKB \u2018Delfia\u2122\u2019 immunofluorometric assay for serum TSH has been evaluated. The assay is simple and rapid to perform and is capable of processing in excess of 100 specimens within a working day. The sensitivity of the assay is 0\u00b705 mU\/L, with a working range extending beyond 324 mU\/L. The change in signal at each point over the standard curve approaches 1000-fold, significantly greater than that of a typical immunoradiometric assay.<\/jats:p>\n                  <jats:p>Mean figures for intra-assay and inter-assay precision were 4\u00b77% and 8\u00b76% CV, respectively. Mean recovery of added TSH was 98\u00b73%, and samples containing high levels of endogenous TSH diluted parallel to the standard curve. The method showed good correlation with an immunoradiometric assay for TSH. The reference ranges for clinically defined groups of subjects were euthyroid 0\u00b747\u20133\u00b784 mU\/L ( n = 83); primary hypothyroidism 15 \u2014 &gt;324 mU\/L ( n = 28); thyrotoxicosis &lt;0\u00b705 mU\/L ( n = 46).<\/jats:p>\n                  <jats:p>It is concluded that the \u2018Delfia\u2019 assay offers the clinical biochemistry laboratory an attractive and reliable alternative to a sensitive immunoradiometric assay for serum TSH.<\/jats:p>","DOI":"10.1177\/000456328502200609","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:57:08Z","timestamp":1391619428000},"page":"606-611","source":"Crossref","is-referenced-by-count":27,"title":["Evaluation of a Time-Resolved Immunofluorometric Assay for Serum Thyroid Stimulating Hormone"],"prefix":"10.1177","volume":"22","author":[{"given":"N","family":"Paterson","sequence":"first","affiliation":[{"name":"Endocrine Section, Department of Clinical Biochemistry, Royal Infirmary, Glasgow G4 0SF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E M","family":"Biggart","sequence":"additional","affiliation":[{"name":"Endocrine Section, Department of Clinical Biochemistry, Royal Infirmary, Glasgow G4 0SF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R S","family":"Chapman","sequence":"additional","affiliation":[{"name":"Endocrine Section, Department of Clinical Biochemistry, Royal Infirmary, Glasgow G4 0SF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G H","family":"Beastall","sequence":"additional","affiliation":[{"name":"Endocrine Section, Department of Clinical Biochemistry, Royal Infirmary, Glasgow G4 0SF, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1985,11,1]]},"reference":[{"key":"bibr1-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.289.6455.1334"},{"key":"bibr2-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(84)90645-7"},{"key":"bibr3-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1038\/305158a0"},{"key":"bibr4-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.1984.tb03446.x"},{"key":"bibr5-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/25.3.353"},{"key":"bibr6-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/29.1.65"},{"key":"bibr7-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.16.5.920-925.1982"},{"key":"bibr8-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1038\/301258a0"},{"key":"bibr9-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/29.10.1777"},{"key":"bibr10-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/29.1.60"},{"key":"bibr11-000456328502200609","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(83)92530-8"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456328502200609","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456328502200609","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:29:02Z","timestamp":1777375742000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/000456328502200609"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,11]]},"references-count":11,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1985,11]]}},"alternative-id":["10.1177\/000456328502200609"],"URL":"https:\/\/doi.org\/10.1177\/000456328502200609","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,11]]}}}