{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:17:31Z","timestamp":1777425451967,"version":"3.51.4"},"reference-count":17,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[1986,9,1]],"date-time":"1986-09-01T00:00:00Z","timestamp":525916800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[1986,9]]},"abstract":"<jats:p>\n                    Simple methods for the detection of hypoxanthine-guanine phosphoribosyltransferase and\/or adenine phosphoribosyltransferase deficiencies using dried filter paper blood spots were studied. Enzyme activities in the eluate from dried filter paper blood spots stored for 4 weeks at room conditions were shown to be quite stable. Autoradiographs prepared from dried filter paper blood spots and DE-81 papers soaked with enzyme reaction mixtures containing\n                    <jats:sup>14<\/jats:sup>\n                    C-hypoxanthine and\/or\n                    <jats:sup>14<\/jats:sup>\n                    C-adenine showed sharp radioactive spots in normal subjects. No activity was evident in the cases of the Lesch-Nyhan syndrome and\/or adenine phosphoribosyltransferase deficiency. The methods seem to be suitable for screening.\n                  <\/jats:p>","DOI":"10.1177\/000456328602300507","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:57:08Z","timestamp":1391619428000},"page":"529-532","source":"Crossref","is-referenced-by-count":3,"title":["Simple Screening Methods for Hypoxanthine-Guanine Phosphoribosyltransferase and Adenine Phosphoribosyltransferase Deficiencies Using Dried Blood Spots on Filter Paper"],"prefix":"10.1177","volume":"23","author":[{"given":"Y","family":"Nishida","sequence":"first","affiliation":[{"name":"Department of Medicine and Physical Therapy, University of Tokyo School of Medicine, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Miyamoto","sequence":"additional","affiliation":[{"name":"Department of Medicine and Physical Therapy, University of Tokyo School of Medicine, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1986,9,1]]},"reference":[{"key":"bibr1-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(64)90104-4"},{"key":"bibr2-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1126\/science.155.3770.1682"},{"key":"bibr3-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.57.6.1735"},{"key":"bibr4-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1042\/bj1570485"},{"key":"bibr5-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-197205000-00004"},{"key":"bibr6-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1007\/BF00484458"},{"key":"bibr7-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198112243052608"},{"key":"bibr8-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1007\/BF00521145"},{"key":"bibr9-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"bibr10-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(84)90291-2"},{"key":"bibr11-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/31.1.151"},{"key":"bibr12-000456328602300507","doi-asserted-by":"publisher","DOI":"10.3109\/00365518109092044"},{"key":"bibr13-000456328602300507","doi-asserted-by":"publisher","DOI":"10.3109\/00365518209168145"},{"key":"bibr14-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(74)80344-6"},{"key":"bibr15-000456328602300507","first-page":"844","volume":"90","author":"Ito K","year":"1977","journal-title":"J Lab Clin Med."},{"key":"bibr16-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(68)80250-1"},{"key":"bibr17-000456328602300507","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(68)80274-4"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456328602300507","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456328602300507","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:29:21Z","timestamp":1777375761000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/000456328602300507"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,9]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1986,9]]}},"alternative-id":["10.1177\/000456328602300507"],"URL":"https:\/\/doi.org\/10.1177\/000456328602300507","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,9]]}}}