{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:04:50Z","timestamp":1777428290146,"version":"3.51.4"},"reference-count":12,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[1989,9,1]],"date-time":"1989-09-01T00:00:00Z","timestamp":620611200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[1989,9]]},"abstract":"<jats:p>A monoclonal antibody (E-21) was obtained that specifically binds to human pancreatic amylase and shows negligible cross-reaction with human salivary amylase. Using this antibody a radioimmunoassay was developed for pancreatic amylase in human serum. The assay was shown to be sensitive (detectable up to 7 mg\/L), reproducible, and specific for pancreatic amylase. In normal subjects, the mean concentration of serum pancreatic amylase determined by this method was 36\u00b73 mg\/L with a 95% confidence range of 16\u00b75 to 79\u00b72 mg\/L. A good correlation was observed between the concentrations of immunoreactive pancreatic amylase (IR-PA) and enzymatic activities in 20 serum samples ( r = 0\u00b797). The concentration of serum IR-PA was below the detectable limit in pancreatectomised patients, and was greatly increased in patients with acute pancreatitis; the latter was accompanied by parallel changes in total enzymic activity. In patients with mumps, the serum IR-PA level was within the normal range whereas the total enzymic activity was elevated.<\/jats:p>","DOI":"10.1177\/000456328902600507","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:57:08Z","timestamp":1391619428000},"page":"416-421","source":"Crossref","is-referenced-by-count":3,"title":["Radioimmunoassay of Human Pancreatic Amylase with Monoclonal Antibody"],"prefix":"10.1177","volume":"26","author":[{"given":"Chiyo","family":"Fujita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chihiro","family":"Kasai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromi","family":"Kosuge","sequence":"additional","affiliation":[{"name":"Eiken Chemical Co. 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