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On that account we have developed a method for measurement of the enzyme activity in human skeletal muscle biopsy material (&gt; 10 mg). Interfering creatine kinase isoenzymes are removed by anion exchange and affinity chromatography. The activity of Mi-CK in reference skeletal muscle homogenates amounts to 240\u00b188 mU\/mg protein (30\u00b18\u00b70 mU\/mg wet weight).<\/jats:p>","DOI":"10.1177\/000456329202900213","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:58:39Z","timestamp":1391619519000},"page":"196-201","source":"Crossref","is-referenced-by-count":17,"title":["A Method for Quantitative Measurement of Mitochondrial Creatine Kinase in Human Skeletal Muscle"],"prefix":"10.1177","volume":"29","author":[{"given":"J","family":"Smeitink","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Wevers","sequence":"additional","affiliation":[{"name":"Institute of Neurology, University Hospital Nijmegen, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Hulshof","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Ruitenbeek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T v","family":"Lith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Sengers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Trijbels","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Korenke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Wallimann","sequence":"additional","affiliation":[{"name":"Institute of Cell Biology, Swiss Federal Institute of Technology, Z\u00fcrich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1992,3,1]]},"reference":[{"key":"bibr1-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(64)90185-8"},{"key":"bibr2-000456329202900213","doi-asserted-by":"crossref","first-page":"16942","DOI":"10.1016\/S0021-9258(18)37482-9","volume":"263","author":"Schlegel J","year":"1988","journal-title":"J Biol Chem"},{"key":"bibr3-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-4723-2_8"},{"key":"bibr4-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)39237-3"},{"key":"bibr5-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1986.61.2.482"},{"key":"bibr6-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1007\/BF00572761"},{"key":"bibr7-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1007\/BF00441735"},{"key":"bibr8-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/21.3.392"},{"key":"bibr9-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)44883-1"},{"key":"bibr10-000456329202900213","first-page":"3","volume-title":"Methods in enzymology","volume":"13","author":"Srere PA","year":"1969"},{"key":"bibr11-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"bibr12-000456329202900213","first-page":"259","volume":"33","author":"Harm K","year":"1987","journal-title":"\u00c4rtzl Lab"},{"key":"bibr13-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(83)90374-1"},{"key":"bibr14-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1159\/000469437"},{"key":"bibr15-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(80)90061-3"},{"key":"bibr16-000456329202900213","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(82)90333-3"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456329202900213","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456329202900213","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:37:21Z","timestamp":1777376241000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/000456329202900213"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,3]]},"references-count":16,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,3]]}},"alternative-id":["10.1177\/000456329202900213"],"URL":"https:\/\/doi.org\/10.1177\/000456329202900213","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,3]]}}}