{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:18:23Z","timestamp":1777429103194,"version":"3.51.4"},"reference-count":21,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1992,7,1]],"date-time":"1992-07-01T00:00:00Z","timestamp":709948800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[1992,7]]},"abstract":"<jats:p>A microtiter plate based Dual Analyte enzymeimmunoassay method for the simultaneous measurement of \u03b1-fetoprotein (AFP) and Free-\u03b2 human chorionic gonadotrophin (hCG) was evaluated. This rapid assay, which has application in both Neural Tube Defect screening and Down's screening, shows good precision with between assay coefficients of variation between 5 and 7\u00b75% for AFP and 3\u00b77 to 5\u00b78% for Free-\u03b2(hCG). Correlation with single analyte procedures is good, with correlation coefficients being greater than 0\u00b791 in both cases. Clinical discrimination in detecting both types of abnormalities is not compromised by this new simultaneous Dual Analyte assay. We conclude that the Dual Analyte approach, which combines analytes achieving the highest known detection efficiency, will bring about improvements in the efficiency of screening, reduce costs and improve report turnaround, all leading to better quality of patient care.<\/jats:p>","DOI":"10.1177\/000456329202900403","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:58:39Z","timestamp":1391619519000},"page":"390-396","source":"Crossref","is-referenced-by-count":23,"title":["Dual Analyte Immunoassay\u2014a New Approach to Neural Tube Defect and Down's Syndrome Screening"],"prefix":"10.1177","volume":"29","author":[{"given":"J N","family":"Macri","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Spencer","sequence":"additional","affiliation":[{"name":"Endocrine Unit, Clinical Biochemistry Department, Oldchurch Hospital, Romford, Essex RM7 0BE, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Anderson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1992,7,1]]},"reference":[{"key":"bibr1-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(84)90530-1"},{"key":"bibr2-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.297.6653.883"},{"key":"bibr3-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970070904"},{"key":"bibr4-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(90)90700-H"},{"key":"bibr5-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1177\/000456329202900504"},{"key":"bibr6-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(90)90980-L"},{"key":"bibr7-000456329202900403","first-page":"468","volume":"303","author":"Macri JN","year":"1991","journal-title":"BMJ"},{"key":"bibr8-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(90)90943-2"},{"key":"bibr9-000456329202900403","first-page":"569","volume":"28","author":"Crossley JA","year":"1991","journal-title":"J Med Genet"},{"key":"bibr10-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/37.6.809"},{"key":"bibr11-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1177\/000456329202900319"},{"key":"bibr12-000456329202900403","first-page":"38","author":"Macri JN","year":"1992","journal-title":"Clin Chem"},{"key":"bibr13-000456329202900403","volume-title":"Immunoassays for Clinical Chemistry","author":"Ekins RP","year":"1983"},{"key":"bibr14-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(72)90766-8"},{"key":"bibr15-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1530\/acta.0.0810487"},{"key":"bibr16-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1177\/000456327701400102"},{"key":"bibr17-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/31.12.2014"},{"key":"bibr18-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/28.7.1469"},{"key":"bibr19-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/27.10.1744"},{"key":"bibr20-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/35.9.1874"},{"key":"bibr21-000456329202900403","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/37.11.1955"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456329202900403","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456329202900403","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:37:32Z","timestamp":1777376252000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/000456329202900403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,7]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,7]]}},"alternative-id":["10.1177\/000456329202900403"],"URL":"https:\/\/doi.org\/10.1177\/000456329202900403","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,7]]}}}