{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:39:11Z","timestamp":1777430351631,"version":"3.51.4"},"reference-count":17,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[1993,7,1]],"date-time":"1993-07-01T00:00:00Z","timestamp":741484800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[1993,7]]},"abstract":"<jats:p>We report a multicentre clinical field trial of a novel dual analyte enzyme immunoassay method for the simultaneous measurement of alpha-fetoprotein (AFP) and free \u03b2-human choriogonadotropin (hCG) in the same microtitre well. The assay was shown to have good technical performance in the hands of all trial centres, with between assay coefficients of variation better than 10% for both analytes across the whole of the assay ranges. The method compared well with single analyte measuring procedures and produced acceptable performance as judged by external quality assurance criteria. Recovery of added analyte and analyte dilution curves also showed acceptable performance.<\/jats:p>\n                  <jats:p>In clinical evaluation of a large set of neural tube defect cases, good clinical discrimination from unaffected cases was observed using APP. With over 150 Down's syndrome cases, the combination of AFP and free (3 hCG confirmed the high detection rates achievable using this marker combination, with detection rates in excess of 70% in early gestation.<\/jats:p>\n                  <jats:p>We conclude that the combination of clinically superior markers coupled with technologically innovative assay design will lead to more efficient Down's screening programmes.<\/jats:p>","DOI":"10.1177\/000456329303000408","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:58:39Z","timestamp":1391619519000},"page":"394-401","source":"Crossref","is-referenced-by-count":26,"title":["Dual Analyte Immunoassay in Neural Tube Defect and Down's Syndrome Screening: Results of a Multicentre Clinical Trial"],"prefix":"10.1177","volume":"30","author":[{"given":"K","family":"Spencer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J N","family":"Macri","sequence":"additional","affiliation":[{"name":"Research Division, NTD Laboratories Inc, Carle Place, New York 11514, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R W","family":"Anderson","sequence":"additional","affiliation":[{"name":"Research Division, NTD Laboratories Inc, Carle Place, New York 11514, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D A","family":"Aitken","sequence":"additional","affiliation":[{"name":"Duncan Guthrie Institute of Medical Genetics, Yorkhill, Glasgow"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Berry","sequence":"additional","affiliation":[{"name":"Duncan Guthrie Institute of Medical Genetics, Yorkhill, Glasgow"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Crossley","sequence":"additional","affiliation":[{"name":"Duncan Guthrie Institute of Medical Genetics, Yorkhill, Glasgow"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P J","family":"Wood","sequence":"additional","affiliation":[{"name":"Regional Endocrine Unit, General Hospital, Tremona Road, Southampton"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E J","family":"Coombes","sequence":"additional","affiliation":[{"name":"Chemical Pathology Department, Queen Alexandra's Hospital, Cosham, Portsmouth"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Stroud","sequence":"additional","affiliation":[{"name":"Chemical Pathology Department, Queen Alexandra's Hospital, Cosham, Portsmouth"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D J","family":"Worthington","sequence":"additional","affiliation":[{"name":"Clinical Chemistry Department, Birmingham Maternity Hospital, Edgbaston, Birmingham"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Doran","sequence":"additional","affiliation":[{"name":"Clinical Chemistry Department, Birmingham Maternity Hospital, Edgbaston, Birmingham"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Barbour","sequence":"additional","affiliation":[{"name":"Clinical Biochemistry Department, Western General Hospital, Edinburgh, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Wilmot","sequence":"additional","affiliation":[{"name":"Clinical Biochemistry Department, Western General Hospital, Edinburgh, UK."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1993,7,1]]},"reference":[{"key":"bibr1-000456329303000408","first-page":"1323","author":"Report of the UK Collaborative Study on alpha fetoprotein in relation to neural tube defects","year":"1972","journal-title":"Lancet"},{"key":"bibr2-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(84)90530-1"},{"key":"bibr3-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(90)90700-H"},{"key":"bibr4-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/37.6.809"},{"key":"bibr5-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1177\/000456329202900319"},{"key":"bibr6-000456329303000408","first-page":"640","volume":"304","author":"Spencer K","year":"1992","journal-title":"Ann Clin Biochem"},{"key":"bibr7-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.304.6827.640-c"},{"key":"bibr8-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92073-O"},{"key":"bibr9-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1177\/000456329202900403"},{"key":"bibr10-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.1987.tb03115.x"},{"key":"bibr11-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1177\/000456329002700506"},{"key":"bibr12-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970101107"},{"key":"bibr13-000456329303000408","volume-title":"Prenatal Diagnosis and Screening.","author":"Wald N","year":"1992"},{"key":"bibr14-000456329303000408","first-page":"107","volume-title":"Proceedings of the ACB National Meeting; 1991","author":"Spencer K","year":"1991"},{"key":"bibr15-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.302.6791.1536-a"},{"key":"bibr16-000456329303000408","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(92)91353-C"},{"key":"bibr17-000456329303000408","first-page":"31","volume":"1","author":"Reynolds TM","year":"1992","journal-title":"Commun Lab Med"}],"container-title":["Annals of Clinical Biochemistry: International Journal of Laboratory Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456329303000408","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/000456329303000408","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T11:42:07Z","timestamp":1777376527000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/000456329303000408"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,7]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1993,7]]}},"alternative-id":["10.1177\/000456329303000408"],"URL":"https:\/\/doi.org\/10.1177\/000456329303000408","relation":{},"ISSN":["0004-5632","1758-1001"],"issn-type":[{"value":"0004-5632","type":"print"},{"value":"1758-1001","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,7]]}}}