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A rapid method is described which will allow the significance of VLDL oxidation to be assessed in clinical studies. VLDL was isolated from heparinized plasma by a 1-h, single spin ultracentrifugation. Total protein was standardized to 25 mg\/L. Oxidation was promoted by the addition of copper ions (17.5 \u03bcmol\/L, final concentration) incubated at 37\u00b0C. Conjugated diene production was followed at 234 nm. Total assay preparation time was 2 h. Urate greatly inhibited the oxidation of VLDL and was successfully removed by size exclusion chromatography. VLDL isolated from frozen plasma (\u221270\u00b0C) was stable for 15 weeks. This simple, rapid method for the isolation of VLDL may be applied to assess the significance of VLDL oxidation in disease.<\/jats:p>","DOI":"10.1177\/000456329803500404","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:58:39Z","timestamp":1391619519000},"page":"504-514","source":"Crossref","is-referenced-by-count":11,"title":["A Simple Method for Assessing Copper-Mediated Oxidation of Very-Low-Density Lipoprotein Isolated by Rapid Ultracentrifugation"],"prefix":"10.1177","volume":"35","author":[{"given":"Jane","family":"McEneny","sequence":"first","affiliation":[{"name":"Department of Clinical Biochemistry, The Queen's University, Institute of Clinical Science, Grosvenor Road, Belfast BT12 6BJ, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elisabeth R","family":"Trimble","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, The Queen's University, Institute of Clinical Science, Grosvenor Road, 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