{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:53:28Z","timestamp":1777431208206,"version":"3.51.4"},"reference-count":14,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[1998,11,1]],"date-time":"1998-11-01T00:00:00Z","timestamp":909878400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Ann Clin Biochem"],"published-print":{"date-parts":[[1998,11]]},"abstract":"<jats:p>\n                    A peroxidase-entrapped and ferrocene-embedded carbon paste (POD-Fc-CP) electrode allows a highly sensitive detection of H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    at levels as low as 10 nmol\/L with practically no interference by coexisting substances, turbidity or coloration of samples. The electrode was applied to the amperometric determination of high-density lipoprotein (HDL)-cholesterol in a very small volume (1\u20132 \u03bcL) using polyethylene glycol (PEG)-modified cholesterol esterase and cholesterol oxidase without prior precipitation or separation of HDL. PEG-modified enzymes exhibit a selective activity toward HDL-cholesterol in the presence of dextran sulphate and MgCl\n                    <jats:sub>2<\/jats:sub>\n                    to generate H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    . The HDL-cholesterol concentrations of human serum samples determined by this method showed a good correlation with those determined by an ordinary spectrophotometric method using PEG-modified enzymes and peroxidase or by a conventional precipitation method.\n                  <\/jats:p>","DOI":"10.1177\/000456329803500606","type":"journal-article","created":{"date-parts":[[2014,2,5]],"date-time":"2014-02-05T16:58:39Z","timestamp":1391619519000},"page":"739-744","source":"Crossref","is-referenced-by-count":11,"title":["Amperometric Determination of High-Density Lipoprotein Cholesterol Using Polyethylene Glycol-Modified Enzymes and a Peroxidase-Entrapped Electrode"],"prefix":"10.1177","volume":"35","author":[{"given":"Hideaki","family":"Kinoshita","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaki","family":"Torimura","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Kano","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tokuji","family":"Ikeda","sequence":"additional","affiliation":[{"name":"Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Sakyo-ku, Kyoto, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1998,11,1]]},"reference":[{"key":"bibr1-000456329803500606","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(77)90874-9"},{"key":"bibr2-000456329803500606","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(75)90125-3"},{"key":"bibr3-000456329803500606","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/31.5.746"},{"key":"bibr4-000456329803500606","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/41.5.717"},{"key":"bibr5-000456329803500606","doi-asserted-by":"publisher","DOI":"10.1002\/elan.1140091603"},{"key":"bibr6-000456329803500606","first-page":"541","volume":"49","author":"Lkeda T","year":"1985","journal-title":"Agric Biol Chem"},{"key":"bibr7-000456329803500606","unstructured":"Usui T, Sasaki T, Kohsada A. 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