{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T21:33:03Z","timestamp":1780090383146,"version":"3.54.0"},"reference-count":29,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p>Enamel Matrix Serine Proteinase 1 (EMSP1) is a proteolytic enzyme that has been isolated from the developing enamel of pig teeth. Its apparent function is to degrade the organic matrix in preparation for enamel maturation. The expression of EMSP1 has never been investigated in another organism besides the pig, and EMSP1 expression in the enamel organ has never been specifically demonstrated in ameloblasts. Here we report the expression of recombinant pig EMSP1 (rpEMSP1), the generation of rabbit polyclonal antibodies against rpEMSPl, the characterization of the antibodies and EMSP1 expression by Western blot and immunohistochemical analyses, the cloning and characterization of a full-length cDNA encoding mouse EMSP1, and the localization of EMSP1 expression in ameloblasts in mouse day 14 first and second molars by in situ hybridization. The full-length mouse EMSP1 cDNA clone has 1237 nucleotides, excluding the poly(A+) tail, and encodes a preproprotein of 255 amino acids. Mouse EMSP1 shares 75% amino acid identity with pig EMSP1 and has three potential N-linked glycosylation sites, two of which are conserved in the pig homologue. Western blot analysis shows that the polyclonal antibodies are specific for EMSP1 and do not cross-react with trypsin. Immunohistochemistry of pig incisors shows discrete staining in the surface enamel at the earliest part of the maturation stage. In mouse molars, in situ hybridization gives a distinct and specific signal in maturation-stage ameloblasts, and in the junctional epithelium following tooth eruption. We conclude that EMSP1 is expressed by pig and mouse ameloblasts during the early maturation stage of amelogenesis.<\/jats:p>","DOI":"10.1177\/00220345000790011301","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"70-76","source":"Crossref","is-referenced-by-count":48,"title":["Localization of EMSP1 Expression During Tooth Formation and Cloning of Mouse cDNA"],"prefix":"10.1177","volume":"79","author":[{"given":"J.C-C.","family":"Hu","sequence":"first","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 7703 Floyd Curl Drive, San Antonio, TX 78284-7888, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.H.","family":"Ryu","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 7703 Floyd Curl Drive, San Antonio, TX 78284-7888, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.J.","family":"Chen","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 7703 Floyd Curl Drive, San Antonio, TX 78284-7888, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Uchida","sequence":"additional","affiliation":[{"name":"Hiroshima University School of Dentistry, Department of Oral Anatomy, 1-2-3, Kasumi, Minami-ku, Hiroshima City, 734-8553 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Wakida","sequence":"additional","affiliation":[{"name":"Hiroshima University School of Dentistry, Department of Oral Anatomy, 1-2-3, Kasumi, Minami-ku, Hiroshima City, 734-8553 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Murakami","sequence":"additional","affiliation":[{"name":"Hiroshima University School of Dentistry, Department of Oral Anatomy, 1-2-3, Kasumi, Minami-ku, Hiroshima City, 734-8553 Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Jiang","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 7703 Floyd Curl Drive, San Antonio, TX 78284-7888, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Q.","family":"Qian","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 7703 Floyd Curl Drive, San Antonio, TX 78284-7888, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 7703 Floyd Curl Drive, San Antonio, TX 78284-7888, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Cttmers","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 7703 Floyd Curl Drive, San Antonio, TX 78284-7888, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.D.","family":"Bartlett","sequence":"additional","affiliation":[{"name":"Department of Biomineralization, Forsyth Dental Center, 140 Fenway, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.P.","family":"Simmer","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, School of Dentistry, Department of Pediatric Dentistry, 7703 Floyd Curl Drive, San Antonio, TX 78284-7888, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2000,1,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02555230"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00296224"},{"key":"atypb3","first-page":"811","volume":"38","author":"Aoba T.","year":"1993","journal-title":"Tanpakushitsu Kakusan Koso"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1177\/10454411990100040101"},{"key":"atypb5","unstructured":"Burgess RC, Maclaren CM (1965). Proteins in developing bovine enamel. In: Tooth enamel I. Stack MV, Fearnhead RW, editors. Bristol: John Wright & Sons Ltd., pp. 74-82."},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1177\/00220345410200020201"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.44.2.332-338.1984"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1016\/0040-8166(74)90030-5"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1177\/00220345790580023701"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.2.857"},{"key":"atypb11","first-page":"233","volume":"24","author":"LeFevre ML","year":"1932","journal-title":"J Am Dent Assoc"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092170202"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1042\/bj2560965"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5320(70)90068-7"},{"key":"atypb15","unstructured":"Robinson C., Kirkham J. (1984). Dynamics of amelogenesis as revealed by protein compositional studies. In: The chemistry and biology of mineralized tissues. Butler WT, editor. Birmingham, AL: Ebsco Media Inc., pp. 248-263."},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.3109\/03008208909114129"},{"key":"atypb17","first-page":"355","volume":"18","author":"Robinson C.","year":"1990","journal-title":"J Biol Buccale"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1177\/08959374960100020901"},{"key":"atypb19","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook J.","year":"1989","edition":"2"},{"key":"atypb20","doi-asserted-by":"publisher","DOI":"10.3109\/03008209809023917"},{"key":"atypb21","doi-asserted-by":"publisher","DOI":"10.1177\/10454411950060020701"},{"key":"atypb22","doi-asserted-by":"publisher","DOI":"10.1177\/00220345980770020601"},{"key":"atypb23","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090020101"},{"key":"atypb24","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091870106"},{"key":"atypb25","doi-asserted-by":"publisher","DOI":"10.1177\/08959374960100020701"},{"key":"atypb26","doi-asserted-by":"publisher","DOI":"10.1007\/BF00334549"},{"key":"atypb27","doi-asserted-by":"publisher","DOI":"10.1007\/BF00315983"},{"key":"atypb28","unstructured":"Witkop CJ Jr, Sauk JJ Jr (1976). Heritable defects of enamel. In: Oral facial genetics. Stewart RE, Prescott GH, editors. St. Louis: C.V. 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