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MUC5B is organized into cysteine-rich N- and C-terminal regions that flank a central tandem-repeat region containing cysteine-rich subdomains and imperfect 29-residue tandem repeats. In earlier work, we have shown that this mucin selectively forms heterotypic complexes with amylase, proline-rich proteins, statherin, and histatins in salivary secretions, and the aim of this study was to identify specific binding domains within MUC5B using the yeast two-hybrid system. Interactions of cysteine-rich domains in the tandem-repeat region (Cysl-Cys4) and C-terminal region (Cys8a, Cys8b, Cys8c) of MUC5B with statherin and histatins were investigated. These studies indicated that histatin 1 selectively bound to Cysl and Cys2, whereas statherin and histatin 1, 3, and 5 selectively bound to Cys8a. Analysis of the primary sequences of the identified binding domains suggests that these domains most probably can fold into globular-like structures in the native mucin. A ProDom blast search revealed that sequences in Cys1, Cys2, and Cys8a exhibit similarity to domains in evolutionarily diverse extracellular proteins known to participate in a wide variety of protein-protein interactions.<\/jats:p>","DOI":"10.1177\/00220345000790020601","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"732-739","source":"Crossref","is-referenced-by-count":31,"title":["Molecular Mapping of Statherin- and Histatin-binding Domains in Human Salivary Mucin MG1 (MUC5B) by the Yeast Two-hybrid System"],"prefix":"10.1177","volume":"79","author":[{"given":"I.","family":"lontcheva","sequence":"first","affiliation":[{"name":"Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.G.","family":"Oppenheim","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.D.","family":"Offner","sequence":"additional","affiliation":[{"name":"Medicine, Boston University School of Medicine, 80 East Concord Street, Boston, MA 02118, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.F.","family":"Troxler","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Department of Periodontology and Oral Biology, Goldman School of Dental Medicine, Boston"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2000,2,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(89)90070-8"},{"key":"atypb2","unstructured":"Cohen RE, Levine MJ (1989). 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