{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T22:55:35Z","timestamp":1785192935198,"version":"3.55.0"},"reference-count":53,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2000,7,1]],"date-time":"2000-07-01T00:00:00Z","timestamp":962409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2000,7]]},"abstract":"<jats:p>The oral cavity is rarely reported to be a site of human immunodeficiency virus (HIV) transmission, despite detectable virus in saliva and relatively frequent prevalence of periodontal disease in HIV-infected persons yielding increased excretion of mononuclear-cell-enriched gingival fluid. To search for possible sources of HIV in saliva, and using the polymerase chain-reaction technique, we sought the presence and shedding patterns of proviral HIV-1 DNA in gingival crevicular fluid in a group of patients previously determined as HIV-1-seropositive. Periodontal status at the collection sites was monitored by several clinical parameters, including Plaque Index, Gingival Index, probing depth, and clinical attachment loss. Gingival crevicular fluid samples were collected by means of paper points. Proviral HIV-1 DNA was detected in the gingival fluid of 17 out of 35 HIV-1-infected patients. Its detection correlated significantly with higher plasma HIV-1 RNA viral load (p = 0.03) and not with peripheral blood CD4+ cell count, the presence of blood in gingival fluid, or oral lesions. There was a significant correlation between clinical attachment loss at the sites of fluid collection and plasma HIV-1 RNA viral load (p = 0.002), and borderline correlation between the latter and probing depth (p = 0.54) in the group of patients harboring proviral HIV-1 DNA in gingival crevicular fluid. The results of our study suggest that mononuclear cells present in gingival crevicular fluid and harboring proviral HIV-1 DNA could represent a potential source of HIV-1 in the presence or absence of local bleeding, especially in persons with advanced HIV infection and increased loss of clinical attachment.<\/jats:p>","DOI":"10.1177\/00220345000790071101","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"1496-1501","source":"Crossref","is-referenced-by-count":45,"title":["Proviral HIV-1 DNA in Gingival Crevicular Fluid of HIV-1-infected Patients in Various Stages of HIV Disease"],"prefix":"10.1177","volume":"79","author":[{"given":"M.","family":"Mati\u010di\u0107","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, University Medical Centre Ljubljana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Poljak","sequence":"additional","affiliation":[{"name":"Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Kramar","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Periodontology, Medical Faculty, University of Ljubljana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Toma\u017ei\u0107","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, University Medical Centre Ljubljana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Vidmar","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, University Medical Centre Ljubljana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Zakotnik","sequence":"additional","affiliation":[{"name":"Institute of Oncology, Ljubljana, Slovenia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U.","family":"Skaleri\u010d","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Periodontology, Medical Faculty, University of Ljubljana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2000,7,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.4.793"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1970.tb01836.x"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1970.tb01837.x"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.66.12.7328-7335.1992"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1992.tb02173.x"},{"issue":"7","key":"atypb6","first-page":"1","volume":"38","author":"Center for Disease Control","year":"1989","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"atypb7","first-page":"1","volume":"41","author":"Center for Disease Control","year":"1992","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"atypb8","first-page":"927","volume":"11","author":"Chebbi F.","year":"1997","journal-title":"AIDS"},{"key":"atypb9","unstructured":"Cimasoni G. (1973). The marginal region. In Gingival crevicular fluid. Myers HM, editor. Basel: S. Karger, pp. 2-8."},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.5.478"},{"key":"atypb11","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1111\/j.1600-0714.1991.tb00900.x","volume":"20","author":"EEC-Clearinghouse on Oral Problems Related to HIV Infection and WHO Collaborating Centre on Oral Manifestations of the Human Immunodeficiency Virus","year":"1991","journal-title":"J Oral Pathol Med"},{"key":"atypb12","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1111\/j.1600-0714.1993.tb01074.x","volume":"22","author":"EEC-Clearinghouse on Oral Problems Related to HIV Infection and WHO Collaborating Centre on Oral Manifestations of the Human Immunodeficiency Virus","year":"1993","journal-title":"J Oral Pathol Med"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.3109\/00016356309028193"},{"key":"atypb14","unstructured":"Ficarra G. (1995). 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