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Cathepsin B was detected with immunostaining. Saliva and dentin cysteine cathepsin and MMP activities on caries lesions were analyzed spectrofluorometrically. Immunostaining demonstrated stronger cathepsins B in carious than in healthy dentin. In carious dentin, cysteine cathepsin activity increased with increasing depth and age in chronic lesions, but decreased with age in active lesions. MMP activity decreased with age in both active and chronic lesions. Salivary MMP activities were higher in patients with active than chronic lesions and with increasing lesion depth, while cysteine cathepsin activities showed no differences. The results indicate that, along with MMPs, cysteine cathepsins are important, especially in active and deep caries.<\/jats:p>","DOI":"10.1177\/0022034510391906","type":"journal-article","created":{"date-parts":[[2011,1,20]],"date-time":"2011-01-20T00:41:41Z","timestamp":1295484101000},"page":"506-511","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":188,"title":["Cysteine Cathepsins in Human Carious Dentin"],"prefix":"10.1177","volume":"90","author":[{"given":"F.D.","family":"Nascimento","sequence":"first","affiliation":[{"name":"Grupo de Estudos em Odontologia, Universidade Bandeirante de S\u00e3o Paulo (UNIBAN), Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.L.","family":"Minciotti","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinar de Investiga\u00e7\u00e3o Bioqu\u00edmica, Universidade de Mogi das Cruzes, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Geraldeli","sequence":"additional","affiliation":[{"name":"The University of Iowa College of Dentistry, Iowa City, IA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.R.","family":"Carrilho","sequence":"additional","affiliation":[{"name":"Grupo de Estudos em Odontologia, Universidade Bandeirante de S\u00e3o Paulo (UNIBAN), Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.H.","family":"Pashley","sequence":"additional","affiliation":[{"name":"School of Dentistry, Medical College of Georgia, Augusta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.R.","family":"Tay","sequence":"additional","affiliation":[{"name":"School of Dentistry, Medical College of Georgia, Augusta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.B.","family":"Nader","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Paulo (UNIFESP), Disciplina de Biologia Molecular, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Salo","sequence":"additional","affiliation":[{"name":"Institute of Dentistry, PO Box 5281, 90014 University of Oulu, Finland"},{"name":"Oulu University Hospital, Oulu, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Tj\u00e4derhane","sequence":"additional","affiliation":[{"name":"Institute of Dentistry, PO Box 5281, 90014 University of Oulu, Finland"},{"name":"Oulu University Hospital, Oulu, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.L.S.","family":"Tersariol","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinar de Investiga\u00e7\u00e3o Bioqu\u00edmica, Universidade de Mogi das Cruzes, S\u00e3o Paulo, Brazil"},{"name":"Universidade Federal de S\u00e3o Paulo (UNIFESP), Disciplina de Biologia Molecular, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,1,19]]},"reference":[{"key":"bibr1-0022034510391906","doi-asserted-by":"publisher","DOI":"10.2310\/6650.2001.33783"},{"key":"bibr2-0022034510391906","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2007.09.012"},{"key":"bibr3-0022034510391906","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.4.2126"},{"key":"bibr4-0022034510391906","doi-asserted-by":"publisher","DOI":"10.1177\/00220345660450053901"},{"key":"bibr5-0022034510391906","doi-asserted-by":"publisher","DOI":"10.1042\/bj2820273"},{"key":"bibr6-0022034510391906","doi-asserted-by":"publisher","DOI":"10.1042\/bj1370387"},{"key":"bibr7-0022034510391906","unstructured":"Buttle DJ (1994). 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