{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:46:21Z","timestamp":1777812381642,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"6","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:p>Enamel biomineralization results in a release of protons into the enamel matrix, causing an acidification of the local microenvironment. This acidification, which may be enhanced by more rapid mineral deposition in the presence of fluoride, must be neutralized by the overlying ameloblasts. The electrogenic sodium bicarbonate co-transporter NBCe1 has been localized in mouse ameloblasts, and has been proposed to have a role in matrix pH regulation. In this study, transcript analysis by PCR showed NBCe1-A present in human ameloblasts, whereas mouse ameloblasts expressed NBCe1-B. In situ hybridization and qPCR in mouse and fetal human incisors showed that NBCe1 mRNA was up-regulated as ameloblasts differentiated. Ingestion of 50 ppm fluoride resulted in an up-regulation of NBCe1 mRNA in maturation-stage mouse ameloblasts in vivo, as compared with controls. NBCe1 expression was up-regulated by low pH, but not by fluoride, in human ameloblast-lineage cells in vitro. The up-regulation of NBCe1 in vivo as enamel maturation and mineralization progressed provides evidence that NBCe1 participates in pH modulation during enamel formation. Up-regulation of NBCe1 in fluorosed maturation ameloblasts in vivo, with no effect of fluoride in vitro, supports the hypothesis that fluoride-enhanced mineral deposition results in acidification of the mineralizing enamel matrix.<\/jats:p>","DOI":"10.1177\/0022034511398273","type":"journal-article","created":{"date-parts":[[2011,3,2]],"date-time":"2011-03-02T02:54:41Z","timestamp":1299034481000},"page":"782-787","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["NBCe1 in Mouse and Human Ameloblasts may be Indirectly Regulated by Fluoride"],"prefix":"10.1177","volume":"90","author":[{"given":"L.","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Orofacial Sciences, University of California, San Francisco, PO Box 0422, 521 Parnassus Avenue, Room S704, San Francisco, CA 94143-0422, USA\u00a0 State Key Laboratory of Oral Diseases, Sichuan University, Chengdu, Sichuan, China\u00a0"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orofacial Sciences, University of California, San Francisco, PO Box 0422, 521 Parnassus Avenue, Room S704, San Francisco, CA 94143-0422, USA\u00a0"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"He","sequence":"additional","affiliation":[{"name":"Department of Orofacial Sciences, University of California, San Francisco, PO Box 0422, 521 Parnassus Avenue, Room S704, San Francisco, CA 94143-0422, USA\u00a0"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pediatric Dentistry, The Institute of Oral Health Science, Samsung Medical Center, and Sungkyunkwan University School of Medicine, Seoul, Korea\u00a0"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Schneider","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, University of California, San Francisco, CA, USA\u00a0"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.L.","family":"Bronckers","sequence":"additional","affiliation":[{"name":"Department of Oral Cell Biology, School of Dentistry ACTA, University of Amsterdam and VU-University of Amsterdam, the Netherlands\u00a0"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.M.","family":"Lyaruu","sequence":"additional","affiliation":[{"name":"Department of Oral Cell Biology, School of Dentistry ACTA, University of Amsterdam and VU-University of Amsterdam, the Netherlands\u00a0"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.K.","family":"DenBesten","sequence":"additional","affiliation":[{"name":"Department of Orofacial Sciences, University of California, San Francisco, PO Box 0422, 521 Parnassus Avenue, Room S704, San Francisco, CA 94143-0422, USA\u00a0  \u00a0Pamela.DenBesten@ucsf.edu"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2011,3,1]]},"reference":[{"key":"atypb1","first-page":"1188","volume":"46","year":"2009","journal-title":"Bone"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509343280"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1177\/0022034509347249"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(84)90171-7"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1177\/00220345850640120701"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M406591200"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M607041200"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00218.2010"},{"key":"atypb9","first-page":"91","volume":"86","year":"2009","journal-title":"Calcif Tissue Int"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.115188"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2007.09.006"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.05.003"},{"key":"atypb13","unstructured":"Nanci\u00a0A,Ten Cate\u00a0AR6th ed.St. Louis, MO: Mosby; 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