{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T01:00:00Z","timestamp":1785200400920,"version":"3.55.0"},"reference-count":30,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2011,9,20]],"date-time":"2011-09-20T00:00:00Z","timestamp":1316476800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p>\n                    Fluid and exocrine secretion of mucins by salivary mucous glands is regulated predominantly by parasympathetic activation of muscarinic receptors. A direct role for subsequent putative signaling steps, phospholipase C (PLC), increased intracellular calcium ([Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    ), and isoforms of protein kinase C (PKC) in mediating muscarinic exocrine secretion has not been elucidated, and these are potential therapeutic targets to enhance mucin secretion in hyposalivary patients. We found that muscarinic-induced mucin secretion by rat sublingual tubulo-acini was dependent upon PLC activation and the subsequent increase in [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    , and further identified a transient PKC-independent component of secretion dependent upon Ca\n                    <jats:sup>2+<\/jats:sup>\n                    release from intracellular stores, whereas sustained secretion required entry of extracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    . Interactions among carbachol, PKC inhibitors, phorbol 12-myristate 13-acetate, and thapsigargin to modulate [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    implicated conventional PKC isoforms in mediating sustained secretion. With increasing times during carbachol perfusion of glands, in situ, PKC-\u03b1 redistributed across glandular membrane compartments and underwent a rapid and persistent accumulation near the luminal borders of mucous cells. PKC-\u03b21 displayed transient localization near luminal borders, whereas the novel PKCs, PKC-\u03b4 or PKC-\u03f5, displayed little or no redistribution in mucous cells. Collective results implicate synergistic interactions between diacylglycerol (DAG) and increasing [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]\n                    <jats:sub>i<\/jats:sub>\n                    levels to activate cPKCs in mediating sustained muscarinic-induced secretion.\n                  <\/jats:p>","DOI":"10.1177\/0022034511422817","type":"journal-article","created":{"date-parts":[[2011,9,20]],"date-time":"2011-09-20T23:14:28Z","timestamp":1316560468000},"page":"1469-1476","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["Role of Calcium and PKC in Salivary Mucous Cell Exocrine Secretion"],"prefix":"10.1177","volume":"90","author":[{"given":"D.J.","family":"Culp","sequence":"first","affiliation":[{"name":"University of Rochester Medical Center, Center for Oral Biology, 601 Elmwood Ave., Rochester, NY 14642, USA"},{"name":"UF College of Dentistry, Department of Oral Biology, PO Box 100424, Gainesville, FL 32610-3003, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Rochester Medical Center, Center for Oral Biology, 601 Elmwood Ave., Rochester, NY 14642, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.L.","family":"Evans","sequence":"additional","affiliation":[{"name":"University of Rochester Medical Center, Center for Oral Biology, 601 Elmwood Ave., Rochester, NY 14642, USA"},{"name":"Unilever Research, Port Sunlight Laboratory, Bebington, Wirral, CH63 3JW, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2011,9,20]]},"reference":[{"key":"bibr1-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1999.276.2.H642"},{"key":"bibr2-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1996.271.6.C1963"},{"key":"bibr3-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.70.113006.100638"},{"key":"bibr4-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.279.3.L429"},{"key":"bibr5-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00151.2002"},{"key":"bibr6-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(67)90179-3"},{"key":"bibr7-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1997.272.1.G31"},{"key":"bibr8-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)90028-4"},{"key":"bibr9-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M008760200"},{"key":"bibr10-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1177\/002215540405200511"},{"key":"bibr11-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201001164"},{"key":"bibr12-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.2001.280.4.C884"},{"key":"bibr13-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00139.2004"},{"key":"bibr14-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)98335-3"},{"key":"bibr15-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(85)90149-4"},{"key":"bibr16-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1016\/B0-12-386860-2\/00629-8"},{"key":"bibr17-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.1991.261.6.G1043"},{"key":"bibr18-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.67.041703.084745"},{"key":"bibr19-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1973.tb04377.x"},{"key":"bibr20-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00158.2003"},{"key":"bibr21-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81763-8"},{"key":"bibr22-0022034511422817","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.070318"},{"key":"bibr23-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)61759-3"},{"key":"bibr24-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400744"},{"key":"bibr25-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.90536.2008"},{"key":"bibr26-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.136465"},{"key":"bibr27-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.57.030195.002555"},{"key":"bibr28-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1994.266.2.C335"},{"key":"bibr29-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(96)00100-8"},{"key":"bibr30-0022034511422817","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(93)90076-I"}],"container-title":["Journal of Dental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0022034511422817","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0022034511422817","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0022034511422817","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:06:25Z","timestamp":1777496785000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0022034511422817"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9,20]]},"references-count":30,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2011,12]]}},"alternative-id":["10.1177\/0022034511422817"],"URL":"https:\/\/doi.org\/10.1177\/0022034511422817","relation":{},"ISSN":["0022-0345","1544-0591"],"issn-type":[{"value":"0022-0345","type":"print"},{"value":"1544-0591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9,20]]}}}