{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T03:55:35Z","timestamp":1787630135818,"version":"build-2736575974"},"reference-count":21,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2012,6,26]],"date-time":"2012-06-26T00:00:00Z","timestamp":1340668800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:p>The success of tissue-engineering therapies is dependent on the ability of scaffolds to guide differentiation of progenitor cells. Here we present a new approach using a biomimetic construct composed of hydroxyapatite modified with an in vitro-derived extracellular matrix (HA-ECM) and seeded with periodontal ligament progenitor cells (PDLCs). The study aimed to investigate the effect of HA-ECM on osteogenic differentiation of PDLCs and in vivo evaluation of the PDLC-seeded HA-ECM constructs using a rat calvarial critical-sized defect model. After flow-cytometric phenotyping of PDLCs for typical mesenchymal stem cell markers, the PDLCs were cultured on HA-ECM or HA alone in osteogenic media and assessed by MTT, alkaline phosphatase (ALP) assays, and real-time qPCR at different time intervals after seeding. New bone formation induced by PDLC-seeded constructs was assessed by histomorphometric analysis at 12 weeks post-operatively. The PDLCs seeded on HA-ECM showed significantly higher ALP activity and up-regulation of bone-related genes. The treatment with PDLC-seeded HA-ECM significantly improved calvarial bone repair, with the highest amount of newly formed bone elicited by cell-seeded constructs cultured for 14 days. Our results highlight the PDLC-seeded HA-ECM constructs as a promising tool for craniofacial bone regeneration.<\/jats:p>","DOI":"10.1177\/0022034512452430","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T11:44:10Z","timestamp":1340797450000},"page":"789-794","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":41,"title":["Bone Repair Using Periodontal Ligament Progenitor Cell-seeded Constructs"],"prefix":"10.1177","volume":"91","author":[{"given":"G.","family":"Tour","sequence":"first","affiliation":[{"name":"Department of Dental Medicine, Karolinska Institutet, Huddinge, P.O. Box 4064, SE-14104, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Wendel","sequence":"additional","affiliation":[{"name":"Department of Dental Medicine, Karolinska Institutet, Huddinge, P.O. Box 4064, SE-14104, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Moll","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Karolinska University Hospital, Huddinge, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Tcacencu","sequence":"additional","affiliation":[{"name":"Department of Dental Medicine, Karolinska Institutet, Huddinge, P.O. 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