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Slices of mandible were cultured for 14 days in the presence or absence of P. gingivalis lipopolysaccharide (LPS) or pro-inflammatory cytokines. Following culture, cell viability and tissue histomorphometry were assessed with quantification of matrix proteins, resident osteoclasts, ligament cells, monocytes, macrophages, and neutrophils. In the absence of inflammatory factors, culture viability, osteoclasts, and matrix components were maintained. LPS or TNF\u03b1 stimulation demonstrated an increase in cellular proliferation, monocyte cells, osteoclast differentiation, and matrix degradation. Pathophysiological bone metabolism can be induced via exposure to LPS and direct influence of TNF\u03b1 within the model despite the absence of systemic circulation, providing a model for inflammatory bone destruction and investigation of the effects of novel therapeutics.<\/jats:p>","DOI":"10.1177\/0022034513495240","type":"journal-article","created":{"date-parts":[[2013,7,15]],"date-time":"2013-07-15T11:50:12Z","timestamp":1373889012000},"page":"728-734","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":21,"title":["A Novel\n                    <i>Ex vivo<\/i>\n                    Culture Model for Inflammatory Bone Destruction"],"prefix":"10.1177","volume":"92","author":[{"given":"A.J.","family":"Sloan","sequence":"first","affiliation":[{"name":"Mineralised Tissue Group, Tissue Engineering and Reparative Dentistry, School of Dentistry, Cardiff University, UK"},{"name":"Cardiff Institute of Tissue Engineering and 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