{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T20:01:54Z","timestamp":1780344114538,"version":"3.54.1"},"reference-count":28,"publisher":"SAGE Publications","issue":"4","license":[{"start":{"date-parts":[[2014,2,19]],"date-time":"2014-02-19T00:00:00Z","timestamp":1392768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>\n                    Autoimmune responses to heat-shock protein 60 (HSP60) contribute to the progression of atherosclerosis, whereas immunization with HSP60 may induce atheroprotective responses. We assessed the capacity of an atheroprotective vaccine that targeted a recombinant HSP60 from Porphyromonas gingivalis (rGroEL) to induce a protective mucosal immune response. Female apolipoprotein E-deficient spontaneously hyperlipidemic (Apoe\n                    <jats:sup>shl<\/jats:sup>\n                    ) mice received sublingual delivery of rGroEL prior to P. gingivalis 381 injection. The animals were euthanized 16 weeks later. Sublingual immunization with rGroEL induced significant rGroEL-specific serum IgG responses. Antigen-specific cells isolated from spleen produced significantly high levels of IL-10 and IFN-\u03b3 after antigen re-stimulation in vitro. Flow cytometric analysis indicated that the frequencies of both IL-10\n                    <jats:sup>+<\/jats:sup>\n                    and IFN-\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    CD4\n                    <jats:sup>+<\/jats:sup>\n                    Foxp3\n                    <jats:sup>+<\/jats:sup>\n                    cells increased significantly in submandibular glands (SMG). Furthermore, sublingual immunization with rGroEL significantly reduced atherosclerosis lesion formation in the aortic sinus and decreased serum CRP, MCP-1, and ox-LDL levels. These findings suggest that sublingual immunization with rGroEL is associated with the increase of IFN\u03b3\n                    <jats:sup>+<\/jats:sup>\n                    or IL-10\n                    <jats:sup>+<\/jats:sup>\n                    Foxp3\n                    <jats:sup>+<\/jats:sup>\n                    cells in SMG and a systemic humoral response, which could be an effective strategy for the prevention of naturally occurring or P. gingivalis-accelerated atherosclerosis.\n                  <\/jats:p>","DOI":"10.1177\/0022034514523784","type":"journal-article","created":{"date-parts":[[2014,2,20]],"date-time":"2014-02-20T01:34:27Z","timestamp":1392860067000},"page":"382-387","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":32,"title":["Sublingual Vaccine with GroEL Attenuates Atherosclerosis"],"prefix":"10.1177","volume":"93","author":[{"given":"M.","family":"Hagiwara","sequence":"first","affiliation":[{"name":"Department of Oral Surgery"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Kurita-Ochiai","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Hashizume-Takizawa","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"Laboratory of Periodontology and Immunology, Division of Oral Science of Health Promotion, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2014,2,19]]},"reference":[{"key":"bibr1-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2005.09.008"},{"key":"bibr2-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2008.01.009"},{"key":"bibr3-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2005.00182.x"},{"key":"bibr4-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2006.02429.x"},{"key":"bibr5-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1016\/j.trim.2012.08.003"},{"key":"bibr6-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.100.4.e20"},{"key":"bibr7-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1038\/ncpcardio1279"},{"key":"bibr8-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(03)00206-8"},{"key":"bibr9-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2010.02432.x"},{"key":"bibr10-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01572-07"},{"key":"bibr11-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.98.4.300"},{"key":"bibr12-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2005.00832.x"},{"key":"bibr13-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-9972(03)00088-0"},{"key":"bibr14-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1159\/000330786"},{"key":"bibr15-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000134378.86443.cd"},{"key":"bibr16-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2008.12.004"},{"key":"bibr17-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2006.00420.x"},{"key":"bibr18-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1186\/ar2838"},{"key":"bibr19-0022034514523784","doi-asserted-by":"publisher","DOI":"10.5551\/jat.10934"},{"key":"bibr20-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.04.010"},{"key":"bibr21-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01216.x"},{"key":"bibr22-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1053\/meta.2003.50001"},{"key":"bibr23-0022034514523784","doi-asserted-by":"publisher","DOI":"10.1161\/ATVBAHA.107.151274"},{"key":"bibr24-0022034514523784","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.10.5986"},{"key":"bibr25-0022034514523784","first-page":"2434","volume":"116","author":"Wang 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