{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:25:11Z","timestamp":1789950311063,"version":"4.0.1"},"reference-count":34,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T00:00:00Z","timestamp":1428883200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:p>The aim of this study was to investigate the response of human periodontal ligament (hPDL) fibroblasts to an intermittent compressive force and its effect on the expression of SOST, POSTN, and TGFB1. A computerized cell compressive force loading apparatus was introduced, and hPDL cells were subjected to intermittent compressive force. The changes in messenger RNA (mRNA) and protein expression were monitored by real-time polymerase chain reaction and Western blot analysis, respectively. An increased expression of SOST, POSTN, and TGFB1 was observed in a time-dependent fashion. Addition of cycloheximide, a transforming growth factor (TGF)\u2013\u03b2 inhibitor (SB431542), or a neutralizing antibody against TGF-\u03b21 attenuated the force-induced expression of SOST and POSTN as well as sclerostin and periostin, indicating a role of TGF-\u03b21 in the pressure-induced expression of these proteins. Enzyme-linked immunosorbent assay analysis revealed an increased level of TGF-\u03b21 in the cell extracts but not in the medium, suggesting that intermittent compressive force promoted the accumulation of TGF-\u03b21 in the cells or their surrounding matrix. In conclusion, an intermittent compressive force regulates SOST\/POSTN expression by hPDL cells via the TGF-\u03b21 signaling pathway. Since these proteins play important roles in the homeostasis of the periodontal tissue, our results indicate the importance of masticatory forces in this process.<\/jats:p>","DOI":"10.1177\/0022034515581372","type":"journal-article","created":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T22:32:42Z","timestamp":1428964362000},"page":"983-989","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":56,"title":["Mechanical Force\u2013induced\n                    <i>TGFB1<\/i>\n                    Increases Expression of\n                    <i>SOST\/POSTN<\/i>\n                    by hPDL Cells"],"prefix":"10.1177","volume":"94","author":[{"given":"J.","family":"Manokawinchoke","sequence":"first","affiliation":[{"name":"Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"},{"name":"Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Limjeerajarus","sequence":"additional","affiliation":[{"name":"Graduate School, Faculty of Engineering, Thai-Nichi Institute of Technology, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Limjeerajarus","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Sastravaha","sequence":"additional","affiliation":[{"name":"Department of Surgery, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V.","family":"Everts","sequence":"additional","affiliation":[{"name":"Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, MOVE Research Institute, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Pavasant","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"},{"name":"Mineralized Tissue Research Unit, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2015,4,13]]},"reference":[{"key":"bibr1-0022034515581372","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2005.04.002"},{"key":"bibr2-0022034515581372","doi-asserted-by":"crossref","unstructured":"Andreasen JO, Kristerson L. 1981. 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