{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,1]],"date-time":"2026-11-01T02:22:48Z","timestamp":1793499768907,"version":"4.5.0"},"reference-count":39,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T00:00:00Z","timestamp":1436227200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:p>\n                    Emerging evidence suggests a role for purinergic signaling in the activation of multiprotein intracellular complexes called inflammasomes, which control the release of potent inflammatory cytokines, such as interleukin (IL) -1\u03b2 and -18. Porphyromonas gingivalis is intimately associated with periodontitis and is currently considered one of the pathogens that can subvert the immune system by limiting the activation of the NLRP3 inflammasome. We recently showed that P. gingivalis can dampen eATP-induced IL-1\u03b2 secretion by means of its fimbriae in a purinergic P2X7 receptor\u2013dependent manner. Here, we further explore the role of this purinergic receptor during eATP-induced IL-1\u03b2 processing and secretion by P. gingivalis\u2013infected macrophages. We found that NLRP3 was necessary for eATP-induced IL-1\u03b2 secretion as well as for caspase 1 activation irrespective of P. gingivalis fimbriae. Additionally, although the secretion of IL-1\u03b2 from P. gingivalis\u2013infected macrophages was dependent on NLRP3, its adaptor protein ASC, or caspase 1, the cleavage of intracellular pro-IL-1\u03b2 to the mature form was found to occur independently of NLRP3, its adaptor protein ASC, or caspase 1. Our in vitro findings revealed that P2X7 receptor has a dual role, being critical not only for eATP-induced IL-1\u03b2 secretion but also for intracellular pro-IL-1\u03b2 processing. These results were relevant in vivo since P2X7 receptor expression was upregulated in a P. gingivalis oral infection model, and reduced IFN-\u03b3 and IL-17 were detected in draining lymph node cells from P2rx7\n                    <jats:sup>-\/-<\/jats:sup>\n                    mice. Furthermore, we demonstrated that P2X7 receptor and NLRP3 transcription were modulated in human chronic periodontitis. Overall, we conclude that the P2X7 receptor has a role in periodontal immunopathogenesis and suggest that targeting of the P2X7\/NLRP3 pathway should be considered in future therapeutic interventions in periodontitis.\n                  <\/jats:p>","DOI":"10.1177\/0022034515593465","type":"journal-article","created":{"date-parts":[[2015,7,7]],"date-time":"2015-07-07T22:10:49Z","timestamp":1436307049000},"page":"1233-1242","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":47,"title":["A Dual Role for P2X7 Receptor during\n                    <i>Porphyromonas gingivalis<\/i>\n                    Infection"],"prefix":"10.1177","volume":"94","author":[{"given":"E.S.","family":"Ramos-Junior","sequence":"first","affiliation":[{"name":"Programa de Imunobiologia, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.C.","family":"Morandini","sequence":"additional","affiliation":[{"name":"Programa de Imunobiologia, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.L.C.","family":"Almeida-da-Silva","sequence":"additional","affiliation":[{"name":"Programa de Imunobiologia, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.J.","family":"Franco","sequence":"additional","affiliation":[{"name":"Department of Periodontology, School of Dentistry, Catholic University of Bras\u00edlia, Bras\u00edlia, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Potempa","sequence":"additional","affiliation":[{"name":"Department of Oral Immunology and Infectious Diseases, University of Louisville School of Dentistry, Louisville, KY, USA; and Department of Microbiology, Faculty of Biochemistry, Biophysics, and Biotechnology, Jagiellonian University, Krakow, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.A.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Faculty of Dentistry, University of Sydney, Sydney, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.C.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Programa de Imunobiologia, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.S.","family":"Zamboni","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, Ribeir\u00e3o Preto Medical School, University of S\u00e3o Paulo, S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.M.","family":"Ojcius","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of the Pacific, Arthur Dugoni School of Dentistry, San Francisco, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Scharfstein","sequence":"additional","affiliation":[{"name":"Programa de Imunobiologia, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Coutinho-Silva","sequence":"additional","affiliation":[{"name":"Programa de Imunobiologia, Instituto de Biof\u00edsica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 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