{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T21:18:21Z","timestamp":1791321501982,"version":"4.3.1"},"reference-count":40,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T00:00:00Z","timestamp":1453248000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:p>\n                    Formation and growth of hydroxyapatite crystals during amelogenesis generate a large number of protons that must be neutralized, presumably by HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    ions transported from ameloblasts into the developing enamel matrix. Ameloblasts express a number of transporters and channels known to be involved in HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    transport in other epithelia. However, to date, there is no functional evidence for HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    transport in these cells. To address questions related to HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    export from ameloblasts, we have developed a polarized 2-dimensional culture system for HAT-7 cells, a rat cell line of ameloblast origin. HAT-7 cells were seeded onto Transwell permeable filters. Transepithelial resistance was measured as a function of time, and the expression of transporters and tight junction proteins was investigated by conventional and quantitative reverse transcription polymerase chain reaction. Intracellular pH regulation and HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    transport were assessed by microfluorometry. HAT-7 cells formed epithelial layers with measureable transepithelial resistance on Transwell permeable supports and expressed claudin-1, claudin-4, and claudin-8\u2014key proteins for tight junction formation. Transport proteins previously described in maturation ameloblasts were also present in HAT-7 cells. Microfluorometry showed that the HAT-7 cells were polarized with a high apical membrane CO\n                    <jats:sub>2<\/jats:sub>\n                    permeability and vigorous basolateral HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    uptake, which was sensitive to Na\n                    <jats:sup>+<\/jats:sup>\n                    withdrawal, to the carbonic anhydrase inhibitor acetazolamide and to H\n                    <jats:sub>2<\/jats:sub>\n                    DIDS inhibition. Measurements of transepithelial HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    transport showed a marked increase in response to Ca\n                    <jats:sup>2+<\/jats:sup>\n                    - and cAMP-mobilizing stimuli. Collectively, 2-dimensional HAT-7 cell cultures on permeable supports 1) form tight junctions, 2) express typical tight junction proteins and electrolyte transporters, 3) are functionally polarized, and 4) can accumulate HCO\n                    <jats:sub>3<\/jats:sub>\n                    <jats:sup>\u2212<\/jats:sup>\n                    ions from the basolateral side and secrete them at the apical membrane. These studies provide evidence for a regulated, vectorial, basolateral-to-apical bicarbonate transport in polarized HAT-7 cells. We therefore propose that the HAT-7 cell line is a useful functional model for studying electrolyte transport by ameloblasts.\n                  <\/jats:p>","DOI":"10.1177\/0022034515625939","type":"journal-article","created":{"date-parts":[[2016,1,20]],"date-time":"2016-01-20T20:51:59Z","timestamp":1453323119000},"page":"588-596","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":34,"title":["Evidence for Bicarbonate Secretion by Ameloblasts in a Novel Cellular Model"],"prefix":"10.1177","volume":"95","author":[{"given":"E.","family":"Bori","sequence":"first","affiliation":[{"name":"Department of Oral Biology, Semmelweis University, Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, MOVE Research Institute, Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"R\u00e1cz","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Semmelweis University, Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Burghardt","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Semmelweis University, Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"F\u00f6ldes","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Semmelweis University, Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Ker\u00e9mi","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Semmelweis University, Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Harada","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Division of Developmental Biology and Regenerative Medicine, Iwate Medical University, Iwate, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.C.","family":"Steward","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences, The University of Manchester, Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Den Besten","sequence":"additional","affiliation":[{"name":"Department of Orofacial Sciences, University of California, San Francisco, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.L.J.J.","family":"Bronckers","sequence":"additional","affiliation":[{"name":"Department of Oral Cell Biology, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, MOVE Research Institute, Amsterdam, Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Varga","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Semmelweis University, Budapest, 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