{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T08:12:28Z","timestamp":1789891948822,"version":"4.0.1"},"reference-count":34,"publisher":"SAGE Publications","issue":"2","license":[{"start":{"date-parts":[[2016,11,16]],"date-time":"2016-11-16T00:00:00Z","timestamp":1479254400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:p>Ionizing radiation is one of the most common cancer treatments; however, the treatment leads to a wide range of debilitating side effects. In patients with head and neck cancer (HNC), the surrounding normal salivary gland is extremely sensitive to therapeutic radiation, and damage to this tissue results in various oral complications and decreased quality of life (QOL). In the current study, mice treated with targeted head and neck radiation showed a significant increase in double-stranded breaks (DSB) in the DNA of parotid salivary gland cells immediately after treatment, and this remained elevated 3 h posttreatment. In contrast, mice pretreated with insulin-like growth factor-1 (IGF-1) showed resolution of the same amount of initial DNA damage by 3 h posttreatment. At acute time points (30 min to 2 h), irradiated parotid glands had significantly decreased levels of the histone deactylase Sirtuin-1 (SirT-1) which has been previously shown to function in DNA repair. Pretreatment with IGF-1 increased SirT-1 protein levels and increased deacetylation of SirT-1 targets involved in DNA repair. Pharmacological inhibition of SirT-1 activity decreased the IGF-1\u2013mediated resolution of DSB. These data suggest that IGF-1 promotes DNA repair in irradiated parotid glands through the maintenance and activation of SirT-1.<\/jats:p>","DOI":"10.1177\/0022034516677529","type":"journal-article","created":{"date-parts":[[2016,11,14]],"date-time":"2016-11-14T22:30:33Z","timestamp":1479162633000},"page":"225-232","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":39,"title":["Insulin-Like Growth Factor-1\u2013Mediated DNA Repair in Irradiated Salivary Glands Is Sirtuin-1 Dependent"],"prefix":"10.1177","volume":"96","author":[{"given":"S.","family":"Meyer","sequence":"first","affiliation":[{"name":"Department of Nutritional Sciences, University of Arizona, Tucson, AZ, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.M.","family":"Chibly","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, University of Arizona, Tucson, AZ, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Burd","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, University of Arizona, Tucson, AZ, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.H.","family":"Limesand","sequence":"additional","affiliation":[{"name":"Department of Nutritional Sciences, University of Arizona, Tucson, AZ, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2016,11,16]]},"reference":[{"key":"bibr1-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.407"},{"key":"bibr2-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2013.42"},{"key":"bibr3-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2008.09.036"},{"key":"bibr4-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2015.07.009"},{"key":"bibr5-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200903033"},{"issue":"4","key":"bibr6-0022034516677529","first-page":"pii.1000180","volume":"4","author":"Chibly AM","year":"2014","journal-title":"J Palliat Care Med"},{"key":"bibr7-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107893"},{"key":"bibr8-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1038\/nature08197"},{"key":"bibr9-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2007.11.007"},{"key":"bibr10-0022034516677529","unstructured":"Hall EJ. (2000). Radiobiology for the radiologist. Philadelphia (PA): Lippincott Williams and Wilkins."},{"key":"bibr11-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.058628"},{"key":"bibr12-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2008.08.001"},{"key":"bibr13-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2008.05.004"},{"key":"bibr14-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1016\/j.ghir.2013.06.001"},{"key":"bibr15-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004663"},{"key":"bibr16-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01846-05"},{"key":"bibr17-0022034516677529","doi-asserted-by":"crossref","unstructured":"Lukas J, Lukas C, Bartek J. 2011. More than just a focus: the chromatin response to DNA damage and its role in genome integrity maintenance. Nat Cell Biol. 13(10):1161\u20131169.","DOI":"10.1038\/ncb2344"},{"key":"bibr18-0022034516677529","doi-asserted-by":"crossref","unstructured":"Mah LJ, El-Osta A, Karagiannis TC. 2010. gammaH2AX: a sensitive molecular marker of DNA damage and repair. Leukemia. 24(4):679\u2013686.","DOI":"10.1038\/leu.2010.6"},{"key":"bibr19-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-1671"},{"key":"bibr20-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0051363"},{"key":"bibr21-0022034516677529","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Redondo P, Vaquero A. 2013. The diversity of histone versus nonhistone sirtuin substrates. Genes Cancer. 4(3\u20134): 148\u2013163.","DOI":"10.1177\/1947601913483767"},{"key":"bibr22-0022034516677529","doi-asserted-by":"crossref","unstructured":"McBurney MW, Clark-Knowles KV, Caron AZ, Gray DA. 2013. SIRT1 is a highly networked protein that mediates the adaptation to chronic physiological stress. Genes Cancer. 4(3\u20134): 125\u2013134.","DOI":"10.1177\/1947601912474893"},{"key":"bibr23-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2010.28"},{"key":"bibr24-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1038\/srep05206"},{"key":"bibr25-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1093\/mutage\/gen039"},{"key":"bibr26-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M414391200"},{"key":"bibr27-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E07-06-0525"},{"key":"bibr28-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.163766"},{"key":"bibr29-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.1.28-38.2006"},{"key":"bibr30-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.2001465"},{"key":"bibr31-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1111\/acel.12219"},{"key":"bibr32-0022034516677529","doi-asserted-by":"crossref","unstructured":"Vissink A, Mitchell JB, Baum BJ, Limesand KH, Jensen SB, Fox PC, Elting LS, Langendijk JA, Coppes RP, Reyland ME. 2010. Clinical management of salivary gland hypofunction and xerostomia in head-and-neck cancer patients: successes and barriers. Int J Radiat Oncol Biol Phys. 78(4):983\u2013991.","DOI":"10.1016\/j.ijrobp.2010.06.052"},{"key":"bibr33-0022034516677529","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.551366"},{"key":"bibr34-0022034516677529","doi-asserted-by":"publisher","DOI":"10.4161\/cc.6.23.5026"}],"container-title":["Journal of Dental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0022034516677529","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/0022034516677529","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/0022034516677529","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:06:46Z","timestamp":1777496806000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/0022034516677529"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,16]]},"references-count":34,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1177\/0022034516677529"],"URL":"https:\/\/doi.org\/10.1177\/0022034516677529","relation":{},"ISSN":["0022-0345","1544-0591"],"issn-type":[{"value":"0022-0345","type":"print"},{"value":"1544-0591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,16]]}}}