{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T11:43:33Z","timestamp":1792496613734,"version":"4.5.0"},"reference-count":29,"publisher":"SAGE Publications","issue":"13","license":[{"start":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T00:00:00Z","timestamp":1571270400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE0235526"],"award-info":[{"award-number":["DE0235526"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE025889"],"award-info":[{"award-number":["DE025889"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE027660"],"award-info":[{"award-number":["DE027660"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2019,12]]},"abstract":"<jats:p>The salivary complex of mammals consists of 3 major pairs of glands: the parotid, submandibular, and sublingual glands. While the 3 glands share similar functional properties, such as saliva secretion, their differences are largely based on the types of secretions they produce. While recent studies have begun to shed light on the underlying molecular differences among the glands, few have examined the global transcriptional repertoire over various stages of gland maturation. To better elucidate the molecular nature of the parotid gland, we have performed RNA sequencing to generate comprehensive and global gene expression profiles of this gland at different stages of maturation. Our transcriptomic characterization and hierarchical clustering analysis with adult organ RNA sequencing data sets has identified a number of molecular players and pathways that are relevant for parotid gland biology. Moreover, our detailed analysis has revealed a unique parotid gland\u2013specific gene signature that may represent important players that could impart parotid gland\u2013specific biological properties. To complement our transcriptomic studies, we have performed single-cell RNA sequencing to map the transcriptomes of parotid epithelial cells. Interrogation of the single-cell transcriptomes revealed the degree of molecular and cellular heterogeneity of the various epithelial cell types within the parotid gland. Moreover, we uncovered a mixed-lineage population of cells that may reflect molecular priming of differentiation potentials. Overall our comprehensive studies provide a powerful tool for the discovery of novel molecular players important in parotid gland biology.<\/jats:p>","DOI":"10.1177\/0022034519882355","type":"journal-article","created":{"date-parts":[[2019,10,17]],"date-time":"2019-10-17T21:02:25Z","timestamp":1571346145000},"page":"1539-1547","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":30,"title":["Transcriptomic and Single-Cell Analysis of the Murine Parotid Gland"],"prefix":"10.1177","volume":"98","author":[{"given":"A.","family":"Oyelakin","sequence":"first","affiliation":[{"name":"Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.A.C.","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.E.","family":"Bard","sequence":"additional","affiliation":[{"name":"Genomics and Bioinformatics Core, State University of New York at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.V.","family":"Kann","sequence":"additional","affiliation":[{"name":"Genomics and Bioinformatics Core, State University of New York at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Horeth","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Smalley","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.M.","family":"Kramer","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Sinha","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.A.","family":"Romano","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo, Buffalo, NY, USA"},{"name":"Department of Biochemistry, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2019,10,17]]},"reference":[{"key":"bibr1-0022034519882355","doi-asserted-by":"crossref","unstructured":"Amano O, Mizobe K, Bando Y, Sakiyama K. 2012. Anatomy and histology of rodent and human major salivary glands: overview of the Japan Salivary Gland Society\u2013sponsored workshop. Acta Histochem Cytochem. 45(5):241\u2013250.","DOI":"10.1267\/ahc.12013"},{"key":"bibr2-0022034519882355","doi-asserted-by":"publisher","DOI":"10.2174\/092986712804143321"},{"key":"bibr3-0022034519882355","doi-asserted-by":"publisher","DOI":"10.1177\/0022034519864592"},{"key":"bibr4-0022034519882355","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2018.08.008"},{"key":"bibr5-0022034519882355","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.22714"},{"key":"bibr6-0022034519882355","doi-asserted-by":"crossref","unstructured":"Denny PC, Ball WD, Redman RS. 1997. Salivary glands: a paradigm for diversity of gland development. 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