{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:55:20Z","timestamp":1777812920625,"version":"3.51.4"},"reference-count":24,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T00:00:00Z","timestamp":1584057600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003977","name":"israel science foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Cabakoff grant foundation"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:p>The current study aimed at investigating the long-term biological mechanisms governing bone regeneration in osseous defects filled with bovine bone (BB). Tooth extraction sockets were filled with BB or left unfilled for natural healing in a C57BL\/6 mouse alveolar regeneration bone model ( n = 12). Seven weeks later, the alveolar bone samples were analyzed histologically with hematoxylin\/eosin and tartrate-resistant acid phosphatase staining. A separate group ( n = 10) was used for RNA sequencing. Osteoclast inhibition was induced by zoledronic acid (ZA) administration at 2 wk postextraction in a third group ( n = 28) for examination of osseous changes and cellular functions with micro\u2013computed tomography and quantitative reverse transcription polymerase chain reaction, respectively. Histological and radiological osseous healing was observed in both BB-filled and normal-healing sockets. However, BB regenerated bone showed significant robust expression of genes associated with bone homeostasis and osteoclasts\u2019 function. Osteoclasts\u2019 inhibition in BB-filled sockets led to decreased bone resorption markers and reduced bone formation to a greater extent than that observed in osteoclasts\u2019 inhibition with natural healing. BB displays long-term biologically active properties, despite a naive osseous histological appearance. These include activation of osteoclasts, which in turn promotes osseous remodeling and maturation of ossified bone.<\/jats:p>","DOI":"10.1177\/0022034520911647","type":"journal-article","created":{"date-parts":[[2020,3,13]],"date-time":"2020-03-13T16:44:57Z","timestamp":1584117897000},"page":"820-829","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":6,"title":["Bovine Bone Promotes Osseous Protection via Osteoclast Activation"],"prefix":"10.1177","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9425-3786","authenticated-orcid":false,"given":"Y.","family":"Klein","sequence":"first","affiliation":[{"name":"Department of Orthodontics, Hadassah Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"},{"name":"Department of Periodontology, Hadassah Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"},{"name":"Department of Biochemistry, Israel\u2013Canada Medical Research Institute, Faculty of Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Shani-Kdoshim","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Hadassah Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Maimon","sequence":"additional","affiliation":[{"name":"The Institute of Dental Sciences, Hadassah Medical Center, Hebrew University, Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Fleissig","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Hadassah Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Levin-Talmor","sequence":"additional","affiliation":[{"name":"The Institute of Dental Sciences, Hadassah Medical Center, Hebrew University, 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