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Understanding the genetic underpinnings of ankyloglossia and cleft palate will be an important step toward rational treatment of these complex anomalies. We inactivated the Sry (sex-determining region Y)\u2013box 2 ( Sox2) gene in the developing oral epithelium, including the periderm, a transient structure that prevents abnormal oral adhesions during development. This resulted in ankyloglossia and cleft palate with 100% penetrance in embryos examined after embryonic day 14.5. In Sox2 conditional knockout embryos, the oral epithelium failed to differentiate, as demonstrated by the lack of keratin 6, a marker of the periderm. Further examination revealed that the adhesion of the tongue and mandible expressed the epithelial markers E-Cad and P63. The expanded epithelia are Sox9-, Pitx2-, and Tbx1-positive cells, which are markers of the dental epithelium; thus, the dental epithelium contributes to the development of oral adhesions. Furthermore, we found that Sox2 is required for palatal shelf extension, as well as for the formation of palatal rugae, which are signaling centers that regulate palatogenesis. In conclusion, the deletion of Sox2 in oral epithelium disrupts palatal shelf extension, palatal rugae formation, tooth development, and periderm formation. The periderm is required to inhibit oral adhesions and ankyloglossia, which is regulated by Sox2. In addition, oral adhesions occur through an expanded dental epithelial layer that inhibits epithelial invagination and incisor development. This process may contribute to dental anomalies due to ankyloglossia.<\/jats:p>","DOI":"10.1177\/0022034520939013","type":"journal-article","created":{"date-parts":[[2020,7,17]],"date-time":"2020-07-17T07:46:57Z","timestamp":1594972017000},"page":"1397-1405","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":14,"title":["<i>Sox2<\/i>\n                    Controls Periderm and Rugae Development to Inhibit Oral Adhesions"],"prefix":"10.1177","volume":"99","author":[{"given":"Y.Y.","family":"Sweat","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology, The University of Iowa, Iowa City, IA, USA"},{"name":"Craniofacial Anomalies Research Center, The University of Iowa, Iowa City, IA, 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