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However, the molecular profile of the jaw osteoblast (OB) remains incomplete. The present study aimed to decipher the bone site-specific profiles of transcription factors (TFs) expressed in OBs in vivo. Using RNA sequencing analysis, we mapped the transcriptome of confirmed OBs from 2 different skeletal sites: mandible (Md) and tibia (Tb). The OB transcriptome contains 709 TF genes: 608 are similarly expressed in Md-OB and Tb-OB, referred to as \u201cOB-core\u201d; 54 TF genes are upregulated in Md-OB, referred to as \u201cMd-set\u201d; and 18 TF genes are upregulated in Tb-OB, referred to as \u201cTb-set.\u201d Notably, the expression of 29 additional TF genes depends on their RNA transcript variants. TF genes with no previously known role in OBs and bone were identified. Bioinformatics analysis combined with review of genetic disease databases and a comprehensive literature search showed a significant contribution of anatomical origin to the OB signatures. Md-set and Tb-set are enriched with site-specific TF genes associated with development and morphogenesis (neural crest vs. mesoderm), and this developmental imprint persists during growth and homeostasis. Jaw and tibia site-specific OB signatures are associated with craniofacial and appendicular skeletal disorders as well as neurocristopathies, dental disorders, and digit malformations. The present study demonstrates the feasibility of a new method to isolate pure OB populations and map their gene expression signature in the context of OB physiological environment, avoiding in vitro culture and its associated biases. Our results provide insights into the site-specific developmental pathways governing OBs and identify new major OB regulators of bone physiology. We also established the importance of the OB transcriptome as a prognostic tool for human rare bone diseases to explore the hidden pathophysiology of craniofacial malformations, among the most prevalent congenital defects in humans.<\/jats:p>","DOI":"10.1177\/00220345221074356","type":"journal-article","created":{"date-parts":[[2022,2,12]],"date-time":"2022-02-12T00:10:06Z","timestamp":1644624606000},"page":"859-869","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":18,"title":["Transcriptional Regulation of Jaw Osteoblasts: Development to Pathology"],"prefix":"10.1177","volume":"101","author":[{"given":"A.","family":"Nassif","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Paris, Dental Faculty, Department of Oral Biology, Paris, France"},{"name":"Centre de Recherche des Cordeliers, Universit\u00e9 de Paris, Sorbonne Universit\u00e9, Inserm, Laboratory of Molecular Oral Pathophysiology, Paris, 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