{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T17:17:21Z","timestamp":1783185441251,"version":"3.54.6"},"reference-count":25,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T00:00:00Z","timestamp":1713139200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"CAMS Innovation Fund for Medical Sciences","award":["2021-I2M-1-052"],"award-info":[{"award-number":["2021-I2M-1-052"]}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:p>The intricate formation of the palate involves a series of complex events, yet its mechanistic basis remains uncertain. To explore major cell populations in the palate and their roles during development, we constructed a spatiotemporal transcription landscape of palatal cells. Palate samples from C57BL\/6 J mice at embryonic days 12.5 (E12.5), 14.5 (E14.5), and 16.5 (E16.5) underwent single-cell RNA sequencing (scRNA-seq) to identify distinct cell subsets. In addition, spatial enhanced resolution omics-sequencing (stereo-seq) was used to characterize the spatial distribution of these subsets. Integrating scRNA-seq and stereo-seq with CellTrek annotated mesenchymal and epithelial cellular components of the palate during development. Furthermore, cellular communication networks between these cell subpopulations were analyzed to discover intercellular signaling during palate development. From the analysis of the middle palate, both mesenchymal and epithelial populations were spatially segregated into 3 domains. The middle palate mesenchymal subpopulations were associated with tooth formation, ossification, and tissue remodeling, with initial state cell populations located proximal to the dental lamina. The nasal epithelium of the palatal shelf exhibited richer humoral immune responses than the oral side. Specific enrichment of Tgf\u03b23 and Pthlh signals in the midline epithelial seam at E14.5 suggested a role in epithelial\u2013mesenchymal transition. In summary, this study provides high-resolution transcriptomic information, contributing to a deeper mechanistic understanding of palate biology and pathophysiology.<\/jats:p>","DOI":"10.1177\/00220345241232317","type":"journal-article","created":{"date-parts":[[2024,4,15]],"date-time":"2024-04-15T07:08:07Z","timestamp":1713164887000},"page":"546-554","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Spatiotemporal Evolution of Developing Palate in Mice"],"prefix":"10.1177","volume":"103","author":[{"given":"B.","family":"Wang","sequence":"first","affiliation":[{"name":"Center for Cleft Lip and Palate Treatment, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Center for Ear Reconstruction, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Zhao","sequence":"additional","affiliation":[{"name":"Center for Cleft Lip and Palate Treatment, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Ma","sequence":"additional","affiliation":[{"name":"Center for Cleft Lip and Palate Treatment, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Cleft Lip and Palate Treatment, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Yin","sequence":"additional","affiliation":[{"name":"Center for Cleft Lip and Palate Treatment, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Song","sequence":"additional","affiliation":[{"name":"Center for Cleft Lip and Palate Treatment, Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,4,15]]},"reference":[{"key":"bibr1-00220345241232317","doi-asserted-by":"publisher","DOI":"10.1159\/000362227"},{"key":"bibr2-00220345241232317","doi-asserted-by":"crossref","unstructured":"Brown NL, Knott L, Halligan E, Yarram SJ, Mansell JP, Sandy JR. 2003. Microarray analysis of murine palatogenesis: temporal expression of genes during normal palate development. Dev Growth Differ. 45(2):153\u2013165.","DOI":"10.1034\/j.1600-0854.2004.00686.x"},{"key":"bibr3-00220345241232317","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03549-5"},{"key":"bibr4-00220345241232317","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.26651"},{"key":"bibr5-00220345241232317","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddv223"},{"key":"bibr6-00220345241232317","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.04.003"},{"key":"bibr7-00220345241232317","doi-asserted-by":"crossref","unstructured":"Cordero DR, Brugmann S, Chu Y, Bajpai R, Jame M, Helms JA. 2011. Cranial neural crest cells on the move: their roles in craniofacial development. 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Diagnostic accuracy of transabdominal ultrasound in detecting prenatal cleft lip and palate: a systematic review. 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