{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T22:59:21Z","timestamp":1792623561152,"version":"4.5.0"},"reference-count":27,"publisher":"SAGE Publications","issue":"13","license":[{"start":{"date-parts":[[2024,6,23]],"date-time":"2024-06-23T00:00:00Z","timestamp":1719100800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1F30DE031149-01"],"award-info":[{"award-number":["1F30DE031149-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["2R35GM119465-06"],"award-info":[{"award-number":["2R35GM119465-06"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R01DE028945-04"],"award-info":[{"award-number":["5R01DE028945-04"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["5R03DE028588-02"],"award-info":[{"award-number":["5R03DE028588-02"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["ZIA HD009015"],"award-info":[{"award-number":["ZIA HD009015"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:p>\n                    Multiple genetic and environmental etiologies contribute to the pathogenesis of cleft palate, which is the most common of the inherited disorders of the craniofacial complex. Insights into the molecular mechanisms regulating osteogenic differentiation and patterning in the palate during embryogenesis are limited and needed for the development of innovative diagnostics and cures. This study used the\n                    <jats:italic>Pax9<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    mouse model with a consistent phenotype of cleft secondary palate to investigate the role of\n                    <jats:italic>Pax9<\/jats:italic>\n                    in the process of palatal osteogenesis. Although prior research has identified the upregulation of Wnt pathway modulators\n                    <jats:italic>Dkk1<\/jats:italic>\n                    and\n                    <jats:italic>Dkk2<\/jats:italic>\n                    in\n                    <jats:italic>Pax9<\/jats:italic>\n                    <jats:sup>-\/-<\/jats:sup>\n                    palate mesenchyme, limitations of spatial resolution and technology restricted a more robust analysis. Here, data from single-nucleus transcriptomics and chromatin accessibility assays validated by in situ highly multiplex targeted single-cell spatial profiling technology suggest a distinct relationship between\n                    <jats:italic>Pax9+<\/jats:italic>\n                    and osteogenic populations. Loss of\n                    <jats:italic>Pax9<\/jats:italic>\n                    results in spatially restricted osteogenic domains bounded by\n                    <jats:italic>Dkk2<\/jats:italic>\n                    , which normally interfaces with\n                    <jats:italic>Pax9<\/jats:italic>\n                    in the mesenchyme. Moreover, the loss of\n                    <jats:italic>Pax9<\/jats:italic>\n                    leads to a disruption in the normal osteodifferentiaion of palatal osteogenic mesenchymal cells. These results suggest that Pax9-dependent Wnt signaling modulators influence osteogenic programming during palate formation, potentially contributing to the observed cleft palate phenotype.\n                  <\/jats:p>","DOI":"10.1177\/00220345241256600","type":"journal-article","created":{"date-parts":[[2024,6,24]],"date-time":"2024-06-24T02:57:35Z","timestamp":1719197855000},"page":"1412-1420","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":13,"title":["Spatial Multi-omics Reveals the Role of the Wnt Modulator, Dkk2, in Palatogenesis"],"prefix":"10.1177","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3387-5770","authenticated-orcid":false,"given":"J.O.","family":"Pi\u00f1a","sequence":"first","affiliation":[{"name":"Section on Craniofacial Genetic Disorders, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA"},{"name":"Department of Biomedical Engineering, University of Utah, Salt Lake City, UT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Raju","sequence":"additional","affiliation":[{"name":"Section on Craniofacial Genetic Disorders, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8156-4681","authenticated-orcid":false,"given":"D.M.","family":"Roth","sequence":"additional","affiliation":[{"name":"Section on Craniofacial Genetic Disorders, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA"},{"name":"School of Dentistry, University of Alberta, Edmonton, AB, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2695-7938","authenticated-orcid":false,"given":"E.W.","family":"Winchester","sequence":"additional","affiliation":[{"name":"University of Connecticut School of Dental Medicine, Farmington, CT, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Padilla","sequence":"additional","affiliation":[{"name":"Molecular Genomics Core, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Iben","sequence":"additional","affiliation":[{"name":"Molecular Genomics Core, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD, 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