{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T06:26:32Z","timestamp":1789194392647,"version":"build-2803163510"},"reference-count":36,"publisher":"SAGE Publications","issue":"11","license":[{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P35037"],"award-info":[{"award-number":["P35037"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:p>Mesenchymal stromal cells (MSCs) are multipotent, progenitor cells that reside in tissues across the human body, including the periodontal ligament (PDL) and gingiva. They are a promising therapeutic tool for various degenerative and inflammatory diseases. However, different heterogeneity levels caused by tissue-to-tissue and donor-to-donor variability, and even intercellular differences within a given MSCs population, restrict their therapeutic potential. There are considerable efforts to decipher these heterogeneity levels using different \u201comics\u201d approaches, including single-cell transcriptomics. Previous studies applied this approach to compare MSCs isolated from various tissues of different individuals, but distinguishing between donor-to-donor and tissue-to-tissue variability is still challenging. In this study, MSCs were isolated from the PDL and gingiva of 5 periodontally healthy individuals and cultured in vitro. A total of 3,844 transcriptomes were generated using single-cell mRNA sequencing. Clustering across the 2 different tissues per donor identified PDL- and gingiva-specific and tissue-spanning MSCs subpopulations with unique upregulated gene sets. Gene\/pathway enrichment and protein-protein interaction (PPI) network analysis revealed differences restricted to several cellular processes between tissue-specific subpopulations, indicating a limited tissue-of-origin variability in MSCs. Gene expression, pathway enrichment, and PPI network analysis across all donors\u2019 PDL- or gingiva-specific subpopulations showed significant but limited donor-to-donor differences. In conclusion, this study demonstrates tissue- and donor-specific variabilities in the transcriptome level of PDL- and gingiva-derived MSCs, which seem restricted to specific cellular processes. Identifying tissue-specific and tissue-spanning subpopulations highlights the intercellular differences in dental tissue\u2013derived MSCs. It could be reasonable to control MSCs at a single-cell level to ensure their properties before transplantation.<\/jats:p>","DOI":"10.1177\/00220345241271997","type":"journal-article","created":{"date-parts":[[2024,9,27]],"date-time":"2024-09-27T01:56:16Z","timestamp":1727402176000},"page":"1141-1152","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":15,"title":["Heterogeneity in Dental Tissue\u2013Derived MSCs Revealed by Single-Cell RNA-seq"],"prefix":"10.1177","volume":"103","author":[{"given":"C.","family":"Behm","sequence":"first","affiliation":[{"name":"Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Mi\u0142ek","sequence":"additional","affiliation":[{"name":"Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Kovar","sequence":"additional","affiliation":[{"name":"Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Derdak","sequence":"additional","affiliation":[{"name":"Core Facilities, Medical University of Vienna, Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"X.","family":"Rausch-Fan","sequence":"additional","affiliation":[{"name":"Clinical Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Austria"},{"name":"Center for Clinical Research, University Clinic of Dentistry, Medical University of Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Moritz","sequence":"additional","affiliation":[{"name":"Clinical Division of Conservative Dentistry and Periodontology, University Clinic of Dentistry, Medical University of Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0485-2142","authenticated-orcid":false,"given":"O.","family":"Andrukhov","sequence":"additional","affiliation":[{"name":"Competence Center for Periodontal Research, University Clinic of Dentistry, Medical University of Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"key":"bibr1-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.19-0535"},{"key":"bibr2-00220345241271997","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v11.i9.604"},{"key":"bibr3-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2018.0132"},{"key":"bibr4-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00094.x"},{"key":"bibr5-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.4096"},{"key":"bibr6-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-020-03600-0"},{"key":"bibr7-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2007.01065.x"},{"key":"bibr8-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.04.048"},{"key":"bibr9-00220345241271997","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2015.2413"},{"key":"bibr10-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1583-4"},{"key":"bibr11-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2021.11.005"},{"key":"bibr12-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba6884"},{"key":"bibr13-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-015-0066-5"},{"key":"bibr14-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-021-02627-9"},{"key":"bibr15-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2010.090566"},{"key":"bibr16-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102405"},{"key":"bibr17-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1995.tb00188.x"},{"key":"bibr18-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1002\/stem.312"},{"key":"bibr19-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-016-1867-3"},{"key":"bibr20-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3192"},{"key":"bibr21-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"issue":"10","key":"bibr22-00220345241271997","first-page":"1066","volume":"98","author":"Sharpe PT","year":"2019","journal-title":"Crit Rev Oral Biol Med"},{"key":"bibr23-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2003.18.4.696"},{"key":"bibr24-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2019.05.031"},{"key":"bibr25-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2019.08.002"},{"key":"bibr26-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3002"},{"key":"bibr27-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.650"},{"key":"bibr28-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12630"},{"key":"bibr29-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1111\/jre.13139"},{"key":"bibr30-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1177\/0022034513497961"},{"key":"bibr31-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-01683-x"},{"key":"bibr32-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1177\/00220345231205283"},{"key":"bibr33-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2022.01.005"},{"key":"bibr34-00220345241271997","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.894008"},{"key":"bibr35-00220345241271997","doi-asserted-by":"publisher","DOI":"10.1177\/0363546519848678"},{"issue":"1","key":"bibr36-00220345241271997","volume":"10","author":"Zhou Y","year":"2019","journal-title":"Nat Commun"}],"container-title":["Journal of Dental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00220345241271997","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/full-xml\/10.1177\/00220345241271997","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00220345241271997","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:08:55Z","timestamp":1777496935000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00220345241271997"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,26]]},"references-count":36,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1177\/00220345241271997"],"URL":"https:\/\/doi.org\/10.1177\/00220345241271997","relation":{},"ISSN":["0022-0345","1544-0591"],"issn-type":[{"value":"0022-0345","type":"print"},{"value":"1544-0591","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,26]]}}}