{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T21:57:29Z","timestamp":1785794249908,"version":"3.56.0"},"reference-count":40,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2024,10,6]],"date-time":"2024-10-06T00:00:00Z","timestamp":1728172800000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CL419\/2-2"],"award-info":[{"award-number":["CL419\/2-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["2272\/20"],"award-info":[{"award-number":["2272\/20"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:p>\n                    Due to its capacity to drive osteoclast differentiation, the receptor activator of nuclear factor kappa-\u03b2 ligand (RANKL) is believed to exert a pathological influence in periodontitis. However, RANKL was initially identified as an activator of dendritic cells (DCs), expressed by T cells, and exhibits diverse effects on the immune system. Hence, it is probable that RANKL, acting as a bridge between the bone and immune systems, plays a more intricate role in periodontitis. Using ligature-induced periodontitis (LIP), rapid alveolar bone loss was detected that was later halted even though the ligature was still present. This late phase of LIP was also linked with immunosuppressive conditions in the gingiva. Further investigation revealed that the ligature prompted an immediate migration of RANK-expressing Langerhans cells (LCs) and EpCAM\n                    <jats:sup>+<\/jats:sup>\n                    DCs, the antigen-presenting cells (APCs) of the gingival epithelium, to the lymph nodes, followed by an expansion of T regulatory (Treg) cells in the gingiva. Subsequently, the ligatured gingiva was repopulated by monocyte-derived RANK-expressing EpCAM\n                    <jats:sup>+<\/jats:sup>\n                    DCs, while gingival epithelial cells upregulated RANKL expression. Blocking RANKL signaling with monoclonal antibodies significantly reduced the frequencies of Treg cells in the gingiva and prevented gingival immunosuppression. In addition, RANKL signaling facilitated the differentiation of LCs from bone marrow precursors. To further investigate the role of RANKL, we used K14-RANKL mice, in which RANKL is overexpressed by gingival epithelial cells. The elevated RANKL expression shifted the steady-state frequencies of LCs and EpCAM\n                    <jats:sup>+<\/jats:sup>\n                    DCs within the epithelium, favoring LCs over EpCAM\n                    <jats:sup>+<\/jats:sup>\n                    DCs. Following ligature placement, heightened levels of Treg cells were observed in the gingiva of K14-RANKL mice, and alveolar bone loss was significantly reduced. These findings suggest that RANKL-RANK interactions between gingival epithelial cells and APCs are crucial for suppressing gingival inflammation, highlighting a protective immunological role for RANKL in periodontitis that was overlooked due to its osteoclastogenic activity.\n                  <\/jats:p>","DOI":"10.1177\/00220345241274370","type":"journal-article","created":{"date-parts":[[2024,10,8]],"date-time":"2024-10-08T08:34:10Z","timestamp":1728376450000},"page":"1281-1290","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":5,"title":["Epithelial RANKL Limits Experimental Periodontitis via Langerhans Cells"],"prefix":"10.1177","volume":"103","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1939-1298","authenticated-orcid":false,"given":"Y.","family":"Netanely","sequence":"first","affiliation":[{"name":"Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Barel","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Naamneh","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Jaber","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Yacoub","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Saba","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Zubeidat","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Saar","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel; Department of Periodontology, Hadassah Medical Center, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Eli-Berchoer","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Yona","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Brand","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Capucha","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Rambam Medical Care Center, Haifa, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Wilensky","sequence":"additional","affiliation":[{"name":"Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel; Department of Periodontology, Hadassah Medical Center, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Loser","sequence":"additional","affiliation":[{"name":"Institute of Immunology, University of Oldenburg, Oldenburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2484-7842","authenticated-orcid":false,"given":"B.E.","family":"Clausen","sequence":"additional","affiliation":[{"name":"Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5118-571X","authenticated-orcid":false,"given":"A.-H.","family":"Hovav","sequence":"additional","affiliation":[{"name":"Institute of Biomedical and Oral Research, Faculty of Dental Medicine, Hebrew University, Jerusalem, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2024,10,6]]},"reference":[{"key":"bibr1-00220345241274370","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2013.05.002"},{"key":"bibr2-00220345241274370","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.2097"},{"key":"bibr3-00220345241274370","doi-asserted-by":"publisher","DOI":"10.1038\/36593"},{"key":"bibr4-00220345241274370","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1116770109"},{"key":"bibr5-00220345241274370","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200501071"},{"key":"bibr6-00220345241274370","doi-asserted-by":"crossref","unstructured":"Brand A, Hovav AH, Clausen BE. 2023. 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