{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:59:24Z","timestamp":1777813164622,"version":"3.51.4"},"reference-count":42,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2025,1,28]],"date-time":"2025-01-28T00:00:00Z","timestamp":1738022400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2016-05477"],"award-info":[{"award-number":["RGPIN-2016-05477"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2023-05145"],"award-info":[{"award-number":["RGPIN-2023-05145"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["NIH grant 5R21DE026839-02"],"award-info":[{"award-number":["NIH grant 5R21DE026839-02"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:p>\n                    The odontoclast is a rarely studied cell type that is overly active in many dental pathologies, leading to tooth loss. It is difficult to find diphyodont mammals in which either physiological or pathological root resorption can be studied. Here we use the adult leopard gecko, which has repeated cycles of physiological tooth resorption and shedding. RNA-seq was carried out to compare gene expression profiles of functional teeth to developing teeth. Genes more highly expressed in bell-stage developing teeth were related to morphogenesis (\n                    <jats:italic>PTHLH<\/jats:italic>\n                    ,\n                    <jats:italic>SFRP2<\/jats:italic>\n                    ,\n                    <jats:italic>SHH<\/jats:italic>\n                    ,\n                    <jats:italic>EDAR<\/jats:italic>\n                    ). Some genes expressed in osteoclasts (\n                    <jats:italic>ACP5<\/jats:italic>\n                    ,\n                    <jats:italic>CTSK<\/jats:italic>\n                    ,\n                    <jats:italic>CSF1R<\/jats:italic>\n                    ) were relatively more abundant in functional teeth compared with developing teeth. There was, however, no differential expression of RANKL (\n                    <jats:italic>TNFSF11<\/jats:italic>\n                    ) in the 2 tooth types. In addition, functional teeth expressed proteolysis genes not found in osteoclasts (\n                    <jats:italic>ADAMTS2<\/jats:italic>\n                    ,\n                    <jats:italic>3<\/jats:italic>\n                    ,\n                    <jats:italic>4<\/jats:italic>\n                    ,\n                    <jats:italic>14; CTSA<\/jats:italic>\n                    ,\n                    <jats:italic>CTSH<\/jats:italic>\n                    ,\n                    <jats:italic>CTSS<\/jats:italic>\n                    ). We used tartrate acid resistant phosphatase and cathepsin K (CTSK) staining to identify odontoclasts in and around the gecko dentition. There were 3 populations of CTSK cells: (1) large, functional multinucleated odontoclasts in the crown of the tooth with a ruffled border inside resorption pits; (2) smaller, precursor cells in the pulp with fewer nuclei; and (3) flattened external precursor cells next to the root and bone of attachment. We found a positive relationship between developing teeth and the population of CTSK+ cells on the root surface. We tested a candidate signal that may be involved in CTSK+ cell presence. An antagonist of CSF1R was delivered to developing teeth in vivo, which resulted in a significant decrease in\n                    <jats:italic>CTSK<\/jats:italic>\n                    and\n                    <jats:italic>CSF1R<\/jats:italic>\n                    compared with DMSO controls. Thus, the CSF1 signaling pathway is upstream of CTSK in teeth. This is the first work to detail the molecular characteristics of odontoclasts during physiological tooth shedding and to demonstrate that in vivo, local drug delivery is possible in the gecko model.\n                  <\/jats:p>","DOI":"10.1177\/00220345241304756","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T13:49:30Z","timestamp":1738158570000},"page":"561-571","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Profiling of Odontoclasts during Physiological Tooth Replacement"],"prefix":"10.1177","volume":"104","author":[{"given":"J.I.","family":"Henriquez","sequence":"first","affiliation":[{"name":"Department of Oral Health Sciences, University of British Columbia, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Flibotte","sequence":"additional","affiliation":[{"name":"Bioinformatics Core, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Oral Health Sciences, University of British Columbia, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1409-8163","authenticated-orcid":false,"given":"J.M.","family":"Richman","sequence":"additional","affiliation":[{"name":"Department of Oral Health Sciences, University of British Columbia, Vancouver, BC, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,1,28]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"crossref","unstructured":"Alyahya L Myers GL. 2021. 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