{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T03:02:36Z","timestamp":1793156556719,"version":"4.5.0"},"reference-count":40,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T00:00:00Z","timestamp":1741737600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:p>\n                    Dental caries, as one of the prevalent oral infectious diseases worldwide, constitutes a considerable disease burden. Fluoride has been widely used to prevent dental caries for decades. However, fluoride alone may not always be sufficient. The major cariogenic bacterial species,\n                    <jats:italic>Streptococcus mutans<\/jats:italic>\n                    , has not been effectively controlled by daily fluoride exposure, possibly because it has a detoxification mechanism. Studies have shown that most microorganisms have fluoride exporters dedicated to exporting fluoride ions (F\n                    <jats:sup>\u2212<\/jats:sup>\n                    ).\n                    <jats:italic>S. mutans<\/jats:italic>\n                    possesses 2 homologous genes,\n                    <jats:italic>\n                      eriC\n                      <jats:sup>F<\/jats:sup>\n                      1\n                    <\/jats:italic>\n                    and\n                    <jats:italic>\n                      eriC\n                      <jats:sup>F<\/jats:sup>\n                      2\n                    <\/jats:italic>\n                    , which encode fluoride exporters, but their function has not been fully clarified. In this work, we constructed the markerless gene deletion mutants, overexpression, and complemented strains of\n                    <jats:italic>S. mutans<\/jats:italic>\n                    UA159. Assessing fluoride sensitivity, intracellular F\n                    <jats:sup>\u2212<\/jats:sup>\n                    levels, and cell membrane permeability revealed that EriC\n                    <jats:sup>F<\/jats:sup>\n                    1 was the major functional unit of the fluoride exporter in\n                    <jats:italic>S. mutans<\/jats:italic>\n                    . To further enhance the antibacterial efficiency of fluoride, we identified 3 diphenylurea derivatives that might target EriC\n                    <jats:sup>F<\/jats:sup>\n                    1 by molecular docking, which significantly enhanced the antibacterial effect of sodium fluoride (NaF) by synergistically impeding fluoride efflux, as demonstrated by chequerboard broth microdilution assays. Moreover, these compounds combined with 1 mM NaF impaired the cariogenicity of\n                    <jats:italic>S. mutans<\/jats:italic>\n                    significantly in vivo and with good biocompatibility, especially compounds 9 and 15. Collectively, these findings suggest that fluoride exporters in\n                    <jats:italic>S. mutans<\/jats:italic>\n                    could serve as a potential target for caries prevention, and the diphenylurea derivatives identified for targeting EriC\n                    <jats:sup>F<\/jats:sup>\n                    1 could be a valuable therapeutic approach when combined with fluoride, providing promising measures for dental caries prevention.\n                  <\/jats:p>","DOI":"10.1177\/00220345251318688","type":"journal-article","created":{"date-parts":[[2025,3,13]],"date-time":"2025-03-13T01:11:56Z","timestamp":1741828316000},"page":"900-909","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecules Targeting EriC\n                    <sup>F<\/sup>\n                    1 Increase\n                    <i>Streptococcus mutans<\/i>\n                    Fluoride Sensitivity"],"prefix":"10.1177","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1771-6120","authenticated-orcid":false,"given":"Q.","family":"Weng","sequence":"first","affiliation":[{"name":"Department of Preventive Dentistry, Shanghai Stomatological Hospital &amp; School of Stomatology, Fudan University, Shanghai, China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry, Shanghai Stomatological Hospital &amp; School of Stomatology, Fudan University, Shanghai, China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Yao","sequence":"additional","affiliation":[{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4247-0134","authenticated-orcid":false,"given":"Q.","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Center for Stomatology &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Gong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Center for Stomatology &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Center for Stomatology &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3410-6300","authenticated-orcid":false,"given":"Y.","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Oral Diseases &amp; National Center for Stomatology &amp; National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry, Shanghai Stomatological Hospital &amp; School of Stomatology, Fudan University, Shanghai, China"},{"name":"Shanghai Key Laboratory of Craniomaxillofacial Development and Diseases, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,3,12]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1215063"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00184-24"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000336397"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-023-01369-z"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10010092"},{"issue":"12","key":"e_1_3_3_7_1","first-page":"241","article-title":"Ten great public health achievements\u2014United States, 1900-1999","volume":"48","author":"Centers for Disease Control and Prevention (CDC)","year":"1999","unstructured":"Centers for Disease Control and Prevention (CDC). 1999. 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