{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:58:51Z","timestamp":1790776731499,"version":"4.1.0"},"reference-count":40,"publisher":"SAGE Publications","issue":"12","license":[{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T00:00:00Z","timestamp":1748822400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:p>\n                    The development of craniofacial bones and teeth relies heavily on the Wnt signaling pathway, yet the specific mechanisms and Wnt variants involved remain under continual investigation. Using publicly available single-cell sequencing data from the mouse incisor, we reveal\n                    <jats:italic toggle=\"yes\">Wnt1<\/jats:italic>\n                    expression across dental structures and investigate its role using a\n                    <jats:italic toggle=\"yes\">Col1a1<\/jats:italic>\n                    -dependent\n                    <jats:italic toggle=\"yes\">Wnt1<\/jats:italic>\n                    transgenic mouse model. Inducing\n                    <jats:italic toggle=\"yes\">Wnt1<\/jats:italic>\n                    early on affects craniofacial bone without disturbing tooth development, but prolonged embryonic induction leads to postnatal mortality with osteopetrosis-like bone overgrowth and malformed teeth. While tooth formation was initially unaffected by postnatal\n                    <jats:italic toggle=\"yes\">Wnt1<\/jats:italic>\n                    induction, prolonged activation impaired tooth root formation and odontoblast differentiation, resulting in shortened roots and thinner dentin. Three-dimensional micro\u2013computed tomography quantification reveal that both embryonic and postnatal activation of\n                    <jats:italic toggle=\"yes\">Wnt1<\/jats:italic>\n                    significantly increase neural crest\u2013derived craniofacial bone volume, whereas mesenchymal-derived craniofacial bones are unaffected. Importantly, osteoclastogenesis is suppressed by\n                    <jats:italic toggle=\"yes\">Wnt1<\/jats:italic>\n                    in a dose-dependent manner, revealed through bulk RNA sequencing and in vitro experiments. These findings emphasize the differential effects of\n                    <jats:italic toggle=\"yes\">Wnt1<\/jats:italic>\n                    on bone development based on origin and highlight its role in modulating osteoclast activity, indicating broader implications for craniofacial development and potential therapeutic avenues.\n                  <\/jats:p>","DOI":"10.1177\/00220345251336191","type":"journal-article","created":{"date-parts":[[2025,6,2]],"date-time":"2025-06-02T06:00:15Z","timestamp":1748844015000},"page":"1415-1424","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":2,"title":["Wnt1\u2019s Differential Effects on Craniofacial Bone and Tooth Development"],"prefix":"10.1177","volume":"104","author":[{"given":"R.","family":"Mahmoud","sequence":"first","affiliation":[{"name":"Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5982-810X","authenticated-orcid":false,"given":"A.","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Luther","sequence":"additional","affiliation":[{"name":"Department of Osteology and Biomechanics, University Medical Center Hamburg, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5750-0753","authenticated-orcid":false,"given":"J.","family":"Pothe","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Nottmeier","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Okine","sequence":"additional","affiliation":[{"name":"Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford University, Palo Alto, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Knauth","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.G.","family":"Lopez","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Bockamp","sequence":"additional","affiliation":[{"name":"Institute for Translational Immunology and Research Center for Immunotherapy, University Medical Center, Johannes Gutenberg University, Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7590-187X","authenticated-orcid":false,"given":"J.","family":"Krivanek","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology, Faculty of Medicine, Masaryk University, Brno, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"LeBlanc","sequence":"additional","affiliation":[{"name":"Centre for Oral, Clinical &amp; Translational Sciences, Faculty of Dentistry, Oral &amp; Craniofacial Sciences, King\u2019s College London, London, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0463-396X","authenticated-orcid":false,"given":"J.","family":"Helms","sequence":"additional","affiliation":[{"name":"Division of Plastic and Reconstructive Surgery, Department of Surgery, Stanford School of Medicine, Stanford University, Palo Alto, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Amling","sequence":"additional","affiliation":[{"name":"Department of Osteology and Biomechanics, University Medical Center Hamburg, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Kaucka","sequence":"additional","affiliation":[{"name":"Max Planck Institute for Evolutionary Biology, Pl\u00f6n, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Schinke","sequence":"additional","affiliation":[{"name":"Department of Osteology and Biomechanics, University Medical Center Hamburg, Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Koehne","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7444-0610","authenticated-orcid":false,"given":"J.","family":"Petersen","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, University of Leipzig Medical Center, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2025,6,2]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2015.07.011"},{"key":"e_1_3_3_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/520064"},{"key":"e_1_3_3_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.067306"},{"key":"e_1_3_3_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2017.00031"},{"key":"e_1_3_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2009.06.001"},{"key":"e_1_3_3_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-385975-4.00008-5"},{"key":"e_1_3_3_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2009.07.015"},{"key":"e_1_3_3_9_1","doi-asserted-by":"publisher","DOI":"10.15252\/embj.201798873"},{"key":"e_1_3_3_10_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.8.2213"},{"key":"e_1_3_3_11_1","volume-title":"Developmental biology","author":"Gilbert SF","year":"2000","unstructured":"Gilbert SF. 2000. 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