{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T13:02:26Z","timestamp":1777813346197,"version":"3.51.4"},"reference-count":39,"publisher":"SAGE Publications","issue":"3","license":[{"start":{"date-parts":[[2025,9,9]],"date-time":"2025-09-09T00:00:00Z","timestamp":1757376000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:p>\n                    Periodontitis, a pervasive chronic inflammatory disorder, is distinguished by the progressive degradation of periodontal tissues and alveolar bone. Despite remarkable progress in understanding the pathogenesis of periodontitis, the involvement of TCR\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    CD4\n                    <jats:sup>\u2212<\/jats:sup>\n                    CD8\n                    <jats:sup>\u2212<\/jats:sup>\n                    T cells, also known as double-negative T (DNT) cells, in the pathophysiology of this disease has not been thoroughly investigated. In this study, we observed a significant reduction in the frequency of TCR\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    DNT cells within the gingival tissues of patients afflicted with periodontitis when compared with healthy individuals. Employing a murine model, we demonstrated that the therapeutic administration of TCR\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    DNT cells resulted in a reduction of alveolar bone resorption and a decrease in inflammatory biomarkers, with the most significant effects observed at lower cell doses. Histological examination and gene expression analysis revealed a notable attenuation in the expression levels of proinflammatory cytokines. Furthermore, transcriptomic profiling elucidated the downregulation of pathways associated with neutrophil activation and interleukin-17 signaling, which are critical in the inflammatory cascade of periodontitis. Both in vitro and in vivo experiments underscored the pivotal role of perforin in TCR\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    DNT cells, which is essential for modulating periodontal inflammation and preventing alveolar bone loss. Collectively, our findings suggest that TCR\u03b1\u03b2\n                    <jats:sup>+<\/jats:sup>\n                    DNT cell therapy may represent a promising novel therapeutic strategy for periodontitis, providing valuable insights into the development of innovative treatment modalities for this prevalent oral health condition.\n                  <\/jats:p>","DOI":"10.1177\/00220345251362744","type":"journal-article","created":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T04:44:51Z","timestamp":1757479491000},"page":"396-406","update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Potential of TCR\u03b1\u03b2\n                    <sup>+<\/sup>\n                    CD4\n                    <sup>-<\/sup>\n                    CD8\n                    <sup>-<\/sup>\n                    T Cells in Periodontitis"],"prefix":"10.1177","volume":"105","author":[{"given":"L.S.","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing, China"},{"name":"Beijing Laboratory of Oral Health, Capital Medical University School of Basic Medicine, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.S.","family":"Shen","sequence":"additional","affiliation":[{"name":"Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou, Guangdong, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.Y.","family":"Huang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing Clinical Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.Y.","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing Clinical Research Institute, Beijing Friendship Hospital, Capital Medical University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of General Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3404-5173","authenticated-orcid":false,"given":"D.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Laboratory of Oral Health, Capital Medical University School of Basic Medicine, Beijing, China"},{"name":"Medical Research Center, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China"},{"name":"Department of Gastroenterology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7435-2637","authenticated-orcid":false,"given":"S.L.","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Laboratory of Oral Health, Capital Medical University School of Basic Medicine, Beijing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2025,9,9]]},"reference":[{"key":"e_1_3_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2013.05.002"},{"key":"e_1_3_2_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.886645"},{"key":"e_1_3_2_4_1","doi-asserted-by":"publisher","DOI":"10.2217\/imt-2016-0137"},{"key":"e_1_3_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(03)00114-6"},{"key":"e_1_3_2_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.18-0157"},{"key":"e_1_3_2_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12002"},{"key":"e_1_3_2_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/cmi.2009.3"},{"key":"e_1_3_2_9_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.65694"},{"key":"e_1_3_2_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2000156"},{"key":"e_1_3_2_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aat0797"},{"key":"e_1_3_2_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2015.136"},{"key":"e_1_3_2_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1302829110"},{"key":"e_1_3_2_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3298"},{"issue":"1","key":"e_1_3_2_15_1","first-page":"181","article-title":"T- and B-cell subsets in periodontitis","volume":"69","author":"Gonzales JR.","unstructured":"Gonzales JR. 2015. 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Prevalence of periodontal disease, its association with systemic diseases and prevention. 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