{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T12:02:04Z","timestamp":1784635324932,"version":"3.55.0"},"reference-count":39,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:00:00Z","timestamp":1784332800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"DOI":"10.13039\/501100003130","name":"fonds wetenschappelijk onderzoek","doi-asserted-by":"publisher","award":["1226325N and G0A2E26N"],"award-info":[{"award-number":["1226325N and G0A2E26N"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"national institutes of health","doi-asserted-by":"publisher","award":["1R01DE031270-01A1"],"award-info":[{"award-number":["1R01DE031270-01A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"abstract":"<jats:p>\n                    The aims of this study were 1) to determine how ligature-induced periodontitis (LIP) disrupts barrier functions of the junctional epithelium (JE); 2) to determine, using a genetic approach, the necessity of Wnt signaling for JE barrier functions; and 3) to test, using a biochemical strategy, whether a WNT therapeutic is sufficient to improve barrier functions of a pocket epithelium. In a murine model of LIP, quantitative analyses performed at multiple time points assessed epithelial apoptosis; expression of attachment proteins laminin 5 and \u03b24 integrin, inflammation, and bone resorption.\n                    <jats:italic toggle=\"yes\">\n                      Axin2\n                      <jats:sup>CreERT2\/+<\/jats:sup>\n                      ; R26R\n                      <jats:sup>mTmG\/+<\/jats:sup>\n                    <\/jats:italic>\n                    mice were used to evaluate how LIP impacted Wnt-responsive cells and their progeny, and\n                    <jats:italic toggle=\"yes\">\n                      K14\n                      <jats:sup>CreERT2\/+<\/jats:sup>\n                      ;Wls\n                      <jats:sup>fl\/fl<\/jats:sup>\n                    <\/jats:italic>\n                    mice were used to determine whether Wnt signaling was required for JE barrier functions. In some cases, LIP was followed by a recovery period to assess molecular changes in pocket epithelium, and in a subset of these mice, a liposomal formulation of human WNT3A protein (L-WNT3A) was tested for its effects on early repair dynamics in pocket epithelium. LIP triggered apoptosis, significantly reduced expression of laminin 5 and \u03b24 integrin in the JE, and disrupted the Wnt-responsive compartment; these epithelial changes were accompanied by inflammation and alveolar bone resorption. Reepithelialization occurred even with a ligature present, but this pocket epithelium had compromised barrier functions. Wntless (Wls) deletion was sufficient to convert a JE into pocket epithelium, while topical L-WNT3A treatment was sufficient to increase hemidesmosomal protein expression in pocket epithelium and reduce inflammation at early time points. LIP destroys barrier functions and thus converts a JE into pocket epithelium. Deletion of epithelial\n                    <jats:italic toggle=\"yes\">Wls<\/jats:italic>\n                    demonstrates that this conversion is a Wnt-dependent event. L-WNT3A restores some early barrier features to pocket epithelium; future studies will focus on the durability of these effects.\n                  <\/jats:p>","DOI":"10.1177\/00220345261458716","type":"journal-article","created":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T11:29:06Z","timestamp":1784374146000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["WNT Signaling Modifies Barrier Functions of Junctional and Pocket Epithelium"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8854-4253","authenticated-orcid":false,"given":"F.","family":"Aellos","sequence":"first","affiliation":[{"name":"Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8063-9431","authenticated-orcid":false,"given":"X.","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology\u2013Head &amp; Neck Surgery, School of Medicine, Indiana University, Indianapolis, IN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Nazari","sequence":"additional","affiliation":[{"name":"Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2321-3995","authenticated-orcid":false,"given":"F.","family":"Hermans","sequence":"additional","affiliation":[{"name":"Department of Cardiology and Organ Systems (COS), Biomedical Research Institute (BIOMED), Faculty of Medicine and Life Sciences, Hasselt University, Diepenbeek, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.M.","family":"Torabi","sequence":"additional","affiliation":[{"name":"Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.C.","family":"Ochweri","sequence":"additional","affiliation":[{"name":"Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Cuevas","sequence":"additional","affiliation":[{"name":"Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA"},{"name":"Department of Diagnostic and Biological Sciences, University of Minnesota School of Dentistry, Minneapolis, MN, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Surgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4565-5222","authenticated-orcid":false,"given":"K.A. 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