{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:58:53Z","timestamp":1790521133445,"version":"4.1.0"},"reference-count":37,"publisher":"SAGE Publications","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"funder":[{"name":"US Department of War grant","award":["W81XWH-21-1-0449"],"award-info":[{"award-number":["W81XWH-21-1-0449"]}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE025866"],"award-info":[{"award-number":["R01DE025866"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["R01DE033991"],"award-info":[{"award-number":["R01DE033991"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.sagepub.com"],"crossmark-restriction":true},"short-container-title":["J Dent Res"],"abstract":"<jats:p>\n                    Dentin, the primary structural component of teeth, is a mineralized tissue formed by odontoblasts, which strongly express the\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    gene.\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    is also expressed in osteocytes, making it a valuable marker for studying bone biology and diseases. Transgenic mouse lines expressing\n                    <jats:italic toggle=\"yes\">Cre<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">CreER<\/jats:italic>\n                    , or fluorescent reporters such as\n                    <jats:italic toggle=\"yes\">GFP<\/jats:italic>\n                    under the control of 8-kb or 10-kb\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    promoters have been widely used to investigate gene functions in odontoblasts and osteocytes. However, the transgenic models exhibit ectopic expression, and reporter signals are often reduced in mature odontoblasts, limiting their utility for lineage tracing and studies of terminal differentiation. To address these limitations, we generated 2 novel knock-in mouse lines,\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    <jats:sup>CreERT2<\/jats:sup>\n                    and\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    <jats:sup>ZsGreen<\/jats:sup>\n                    , using CRISPR-Cas9\u2013mediated genome editing. In both models, an internal ribosome entry site (\n                    <jats:italic toggle=\"yes\">IRES<\/jats:italic>\n                    )\u2013\n                    <jats:italic toggle=\"yes\">CreERT2<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">IRES-ZsGreen<\/jats:italic>\n                    cassette was inserted into the 3\u2032 untranslated region (UTR) of the endogenous\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    locus. This design preserves normal\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    expression while permitting robust,\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    -specific expression of\n                    <jats:italic toggle=\"yes\">CreERT2<\/jats:italic>\n                    or\n                    <jats:italic toggle=\"yes\">ZsGreen<\/jats:italic>\n                    in odontoblasts and cementoblast\/cementocytes. By maintaining endogenous regulatory control, these knock-in lines eliminate the ectopic transgene expression commonly observed in transgenic mice generated by injecting the promoter-driven constructs. The\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    <jats:sup>ZsGreen<\/jats:sup>\n                    line enables direct visualization of\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    -expressing odontoblasts and cementoblasts\/cementocytes, while the\n                    <jats:italic toggle=\"yes\">Dmp1<\/jats:italic>\n                    <jats:sup>CreERT2<\/jats:sup>\n                    line allows inducible genetic manipulation in these cells in vivo. Together, these models provide faithful tools for investigating the cellular and molecular mechanisms regulating dentinogenesis and osteocyte biology, as well as for studying dental and alveolar bone tissues, with potential applications in craniofacial biology.\n                  <\/jats:p>","DOI":"10.1177\/00220345261478577","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:43:20Z","timestamp":1790243000000},"update-policy":"https:\/\/doi.org\/10.1177\/sage-journals-update-policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Dmp1<\/i>\n                    Knock-in Mice Faithfully Report and Manipulate\n                    <i>Dmp1<\/i>\n                    -Expressing Cells"],"prefix":"10.1177","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-6546-6643","authenticated-orcid":false,"given":"T.H.","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Developmental Biology, Harvard School of Dental Medicine, Harvard Stem Cell Institute, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Harvard School of Dental Medicine, Harvard Stem Cell Institute, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8922-9976","authenticated-orcid":false,"given":"Y.","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Harvard School of Dental Medicine, Harvard Stem Cell Institute, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Harvard School of Dental Medicine, Harvard Stem Cell Institute, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.J.","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Harvard School of Dental Medicine, Harvard Stem Cell Institute, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3933-887X","authenticated-orcid":false,"given":"Y.","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Developmental Biology, Harvard School of Dental Medicine, Harvard Stem Cell Institute, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"e_1_3_3_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11248-019-00177-8"},{"key":"e_1_3_3_3_1","doi-asserted-by":"crossref","unstructured":"Arhar A Pavli\u010d A Ho\u010devar L. 2024. 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