{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:14:52Z","timestamp":1777810492621,"version":"3.51.4"},"reference-count":26,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[1990,10,1]],"date-time":"1990-10-01T00:00:00Z","timestamp":654739200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[1990,10]]},"abstract":"<jats:p>Eleven children, each having one or two pairs of premolars to be extracted for orthodontic purposes, participated in the study. The model involved placement of a special orthodontic band that allowed the accumulation of plaque in a defined area between the band and the buccal enamel. Examination of enamel changes was carried out in experimental teeth that had been exposed to local plaque accumulation for one, two, four, eight, or 14 days. The specimens were examined under the light (LM) and the scanning electron microscope (SEM). All teeth had signs of very mild dental fluorosis. No indications of demineralization were noted after one day. SEM examination showed signs of crystal dissolution in some of the two-day specimens. Six of eight four-day specimens exhibited surface dissolution. All eight- and 14-day specimens showed signs of surface demineralization in the LM as well as in the SEM. These observations documented that undisturbed bacterial deposits are capable of initiating enamel demineralization within short time periods, even in children living in a water-fluoridated area.<\/jats:p>","DOI":"10.1177\/00220345900690100101","type":"journal-article","created":{"date-parts":[[2010,11,28]],"date-time":"2010-11-28T21:14:56Z","timestamp":1290978896000},"page":"1626-1633","source":"Crossref","is-referenced-by-count":6,"title":["A Light and Scanning Electron Microscopic Study of Enamel Decalcification in Children Living in a Water-fluoridated Area"],"prefix":"10.1177","volume":"69","author":[{"given":"A.","family":"Thylstrup","sequence":"first","affiliation":[{"name":"Department of Structural Properties of Materials The Technical University of Denmark, DK-2800 Lyngby, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.M.","family":"Boyar","sequence":"additional","affiliation":[{"name":"Department of Stomatology and Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, Canada, R3E OW2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Holmen","sequence":"additional","affiliation":[{"name":"Department of Cariology and Endodontics, Royal Dental College, N\u00f8rre All\u00e9 20, DK-2200 Copenhagen N, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.H.","family":"Bowden","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, Faculty of Dentistry, University of Manitoba, Winnipeg, Canada, R3E OW2"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1990,10,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345840630100501"},{"key":"atypb2","first-page":"193","volume":"94","author":"Artun, J.","year":"1986","journal-title":"Scand J Dent Res"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1016\/0889-5406(89)90166-2"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1177\/00220345890680120301"},{"key":"atypb5","doi-asserted-by":"crossref","unstructured":"Brown, W.E. and Konig, K.G., Eds. (1977): Cariostatic Mechanisms of Fluoride, Caries Res 11 (Suppl 1).","DOI":"10.1159\/000260298"},{"key":"atypb6","volume-title":"Mineral Aspects of Dentistry, Monogr Oral Sci, Vol. 10","author":"Driessens, F.C.M.","year":"1982"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1159\/000260293"},{"key":"atypb8","unstructured":"Fejerskov, O. and Thylstrup, A. (1986): Dental Enamel. In: Human Oral Embryology and Histology, LA. Mjor and O. Fejerskov, Eds. Copenhagen: Munksgaard, pp. 50-88."},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1159\/000260304"},{"key":"atypb10","first-page":"2019","volume":"1","author":"Holmen, L.","year":"1987","journal-title":"Scanning Microsc"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1159\/000261116"},{"key":"atypb12","unstructured":"Holmen, L. and Thylstrup, A. (1986): Natural Caries Development and Its Arrestment . In: Factors Relating to Demineralisation and Remineralisation of the Teeth, S.A. Leach, Ed. Oxford: IRL Press Ltd, pp. 139-152."},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1159\/000261065"},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.3109\/00016358709096362"},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1159\/000260866"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1159\/000260867"},{"key":"atypb17","first-page":"149","volume":"14","author":"Houwink, B.","year":"1966","journal-title":"Adv Fluorine Res Dent Caries Prevent"},{"key":"atypb18","volume-title":"Emaljens Opl\u00f8selighed, Caries og Erosioner, Thesis","author":"Larsen, M.J.","year":"1975"},{"key":"atypb19","volume-title":"Oral Microbiology, Sunbury on Thames: Nelson","author":"Marsh, P.D.","year":"1984"},{"key":"atypb20","unstructured":"McCann, H.G. (1968): Inorganic Components of Saliva Secretions. In: Art and Science of Dental Caries Research, R.S. Harris, Ed. New York: Academic Press, pp. 55-73."},{"key":"atypb21","unstructured":"M\u00f8ller, I.J. (1965): Dental Fluorose og Caries. En Unders\u00f8gelse over Litteraturen samt nogle Epidemiologiske Unders\u00f8gelser af Danske B\u00f8rn. Thesis , Copenhagen:Rhodos, pp. 124-151."},{"key":"atypb22","unstructured":"Nancollas, G.H. (1983): Kinetics of Demineralisation and Remineralisation . In: Demineralisation and Remineralisation of the Teeth, S.A. Leach and W.M. Edgar, Eds. Oxford: IRL Press Ltd., pp. 113-128."},{"key":"atypb23","volume-title":"Understanding Dental Caries. 1. Etiology and Mechanisms. 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