{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:26:36Z","timestamp":1777811196772,"version":"3.51.4"},"reference-count":47,"publisher":"SAGE Publications","issue":"5","license":[{"start":{"date-parts":[[1993,5,1]],"date-time":"1993-05-01T00:00:00Z","timestamp":736214400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[1993,5]]},"abstract":"<jats:p>Weanling specific pathogen-free Osborne-Mendel rats were fed a high-calcium, high-phosphorus diet with various levels of sucrose and inoculated with Streptococcus sobrinus strain 6715-13WT and Actinomyces viscosus strain OMZ-105 in order to determine whether calculus and caries could develop simultaneously. Rats consumed diets designated RC-16-5, RC-16-25, or RC-16-50 which partially replaced the corn starch component with progressively higher levels of sucrose, thus, to 5, 25, or 50% sucrose. In general, bacterial recoveries of A. viscosus declined with higher sucrose content of the diet, but a pattern of recovery for S. sobrinus was less clear with respect to dietary sucrose. S. sobrinus, however, was recovered at higher percentages from the tooth surface flora at the later two of three sampling dates. Most calculus-identified by the brittle quality, staining characteristics, and apatitic x-ray diffraction patterns of tooth surface deposits-was formed on the maxillary molars, and most carious lesions occurred on mandibular molars. While there was minimal association of the calculus score with the amount of sucrose in the diet, calculus scores increased greatly from 23 to 43 days after infectious challenge. Caries scores, of both fissure and smooth surfaces, by contrast, increased in a dose-response fashion with increasing dietary sucrose and with time. It is thus possible to induce calculus formation and caries simultaneously in specific pathogen-free Osborne-Mendel rats consuming a high-calcium and -phosphorus diet conducive to calculus formation and containing sucrose. This model appears to be well-suited for simultaneous evaluation of the putative calculus-inhibitory and caries-inhibitory effects of oral therapeutic agents.<\/jats:p>","DOI":"10.1177\/00220345930720050501","type":"journal-article","created":{"date-parts":[[2010,11,28]],"date-time":"2010-11-28T21:14:56Z","timestamp":1290978896000},"page":"858-864","source":"Crossref","is-referenced-by-count":3,"title":["Simultaneous Caries Induction and Calculus Formation in Rats"],"prefix":"10.1177","volume":"72","author":[{"given":"J.M.","family":"Tanzer","sequence":"first","affiliation":[{"name":"School of Dental Medicine, School of Medicine, University of Connecticut, Farmington, Connecticut 06030-1605"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.P.","family":"Grant","sequence":"additional","affiliation":[{"name":"School of Dental Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"McMahon","sequence":"additional","affiliation":[{"name":"School of Dental Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Clinton","sequence":"additional","affiliation":[{"name":"School of Dental Medicine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.D.","family":"Eanes","sequence":"additional","affiliation":[{"name":"National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1993,5,1]]},"reference":[{"key":"atypb1","first-page":"30","volume":"66","author":"Ainamo J.","year":"1970","journal-title":"Suomen Hammaslaakariseuran Tomituksia"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1971.42.1.21"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1177\/00220345590380062601"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1961.32.3.190"},{"key":"atypb5","unstructured":"Carranza FA Jr (1984). 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