{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:32:26Z","timestamp":1777811546334,"version":"3.51.4"},"reference-count":43,"publisher":"SAGE Publications","issue":"8","license":[{"start":{"date-parts":[[1994,8,1]],"date-time":"1994-08-01T00:00:00Z","timestamp":775699200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[1994,8]]},"abstract":"<jats:p>\n                    Activation of the protein kinase C (PKC)-related signal transduction system has been associated with phenotypic expression in a wide variety of cell types. In in vitro studies, it has often been activated by relatively small increases in the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    concentration ([Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ]) in the medium. The studies reported here explored the hypothesis that localized increases in the extracellular [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ] and activation of the PKC-related pathway may be involved in early dentin and enamel formation. Whole-head, freeze-dried sections through the developing molars of 5-day-old rats were evaluated by methods that localized non-crystalline Ca\n                    <jats:sup>2+<\/jats:sup>\n                    . Immunohistochemical methods were adapted for use with the freeze-dried sections, and two monoclonal antibodies were used to localize PKCa in the formative cells of the developing teeth.\n                  <\/jats:p>\n                  <jats:p>\n                    Low concentrations of extracellular Ca\n                    <jats:sup>2+<\/jats:sup>\n                    were observed in the early, unmineralized dentin in the area of ameloblast differentiation. Increased concentrations occurred at the point of initial dentin mineralization, immediately before the beginning of enamel matrix deposition. PKCa was localized in the differentiating odontoblasts, at the beginning of dentin matrix deposition. It was intensely localized in the distal borders of the pre-ameloblasts, and appeared to redistribute in the cells during ameloblast differentiation. These observations suggest that local increases in the extracellular [Ca\n                    <jats:sup>2+<\/jats:sup>\n                    ] and the PKC signal transduction pathway may be involved in key inductions in the early stages of dentin and enamel formation.\n                  <\/jats:p>","DOI":"10.1177\/00220345940730080601","type":"journal-article","created":{"date-parts":[[2017,7,19]],"date-time":"2017-07-19T01:06:45Z","timestamp":1500426405000},"page":"1429-1436","source":"Crossref","is-referenced-by-count":14,"title":["Distribution of Protein Kinase Ca and Accumulation of Extracellular Ca2+ During Early Dentin and Enamel Formation"],"prefix":"10.1177","volume":"73","author":[{"given":"J.W.","family":"Bawden","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry, School of Dentistry, University of North Carolina, Chapel Hill, North Carolina 27599-7450"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Rozell","sequence":"additional","affiliation":[{"name":"The Center for Oral Biology, Karolinska Institute, P.O. Box 4064, S-141 04 Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Wurtz","sequence":"additional","affiliation":[{"name":"The Center for Oral Biology, Karolinska Institute, P.O. Box 4064, S-141 04 Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Fouda","sequence":"additional","affiliation":[{"name":"The Center for Oral Biology, Karolinska Institute, P.O. Box 4064, S-141 04 Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Hammarstrom","sequence":"additional","affiliation":[{"name":"The Center for Oral Biology, Karolinska Institute, P.O. Box 4064, S-141 04 Huddinge, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1994,8,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/S0031-6997(25)06865-6","volume":"38","author":"Abdel-Latif AA","year":"1986","journal-title":"Pharmacol Rev"},{"key":"atypb2","first-page":"245","volume":"68","author":"Argentino L.","year":"1993","journal-title":"Boll Soc Ital Biol Sper"},{"key":"atypb3","doi-asserted-by":"crossref","first-page":"21086","DOI":"10.1016\/S0021-9258(17)45330-0","volume":"265","author":"Berghard A.","year":"1990","journal-title":"J Biol Chem"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1038\/341197a0"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1993.46"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.2.14.2972578"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.4.1273"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.115.4.1171"},{"key":"atypb9","first-page":"1103","volume":"1","author":"Bringas P.","year":"1987","journal-title":"Scanning Electron Microsc"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.10.3.1164"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.119.2.313"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(92)90010-6"},{"key":"atypb13","unstructured":"Gomori G. (1961). The calcium-cobalt method for alkaline phosphatase . In: Histochemistry, theoretical and applied. Pearse AG, editor. London: Churchill, pp 868-869."},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1007\/BF02008094"},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1126\/science.3291115"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.8.3195"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/9.6.1033"},{"key":"atypb18","first-page":"321","volume":"18","author":"Josephsen K.","year":"1990","journal-title":"J Biol Buccale"},{"key":"atypb19","doi-asserted-by":"publisher","DOI":"10.3109\/10520296409063307"},{"key":"atypb20","first-page":"200","volume":"70","author":"Kashiwa HK","year":"1970","journal-title":"Clin Orthop Rel Res"},{"key":"atypb21","unstructured":"Kollar EJ (1983). Epithelial-mesenchymal interaction in the mammalian integument: tooth development as a model for interactive induction. In: Epithelial-mesenchymal interaction in development. Sawyer RH, Falcon JF, editors. New York: Praeger Publishers, pp 27-56."},{"key":"atypb22","doi-asserted-by":"publisher","DOI":"10.1016\/0169-6009(92)90873-C"},{"key":"atypb23","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"atypb24","doi-asserted-by":"crossref","first-page":"13223","DOI":"10.1016\/S0021-9258(18)37695-6","volume":"263","author":"Leach K.","year":"1988","journal-title":"J Biol Chem"},{"key":"atypb25","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.6.3.1740241"},{"key":"atypb26","doi-asserted-by":"publisher","DOI":"10.1038\/308693a0"},{"key":"atypb27","doi-asserted-by":"publisher","DOI":"10.1126\/science.1411571"},{"key":"atypb28","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041460113"},{"key":"atypb29","unstructured":"Ruch JV ( 1985). Epithelial-mesenchymal interactions. In: The chemistry and biology of mineralized connective tissues. Butler W, editor. Birmingham: Ebsco Media, Inc., pp 54-62."},{"key":"atypb30","first-page":"309","volume":"255","author":"Siess W.","year":"1988","journal-title":"Biochem J"},{"key":"atypb31","doi-asserted-by":"publisher","DOI":"10.1177\/00220345880670090101"},{"key":"atypb32","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.1320310508"},{"key":"atypb33","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(91)90515-V"},{"key":"atypb34","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1992.263.1.C130"},{"key":"atypb35","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1387\/ijdb.2485706","volume":"33","author":"Thesleff I.","year":"1989","journal-title":"Int J Dev Biol"},{"key":"atypb36","first-page":"229","volume":"11","author":"Thesleff I.","year":"1991","journal-title":"J Craniofac Genet Dev Biol"},{"key":"atypb37","first-page":"118","author":"Ullberg S.","year":"1954","journal-title":"Acta Radiol (Suppl)"},{"key":"atypb38","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1083\/jcb.112.6.1241","volume":"112","author":"Usuda N.","year":"1991","journal-title":"J Cell Biol"},{"key":"atypb39","doi-asserted-by":"publisher","DOI":"10.1038\/360741a0"},{"key":"atypb40","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1091790403"},{"key":"atypb41","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.117.1.121"},{"key":"atypb42","doi-asserted-by":"publisher","DOI":"10.1038\/317546a0"},{"key":"atypb43","doi-asserted-by":"publisher","DOI":"10.1177\/08959374870010020801"}],"container-title":["Journal of Dental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00220345940730080601","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00220345940730080601","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:03:15Z","timestamp":1777496595000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00220345940730080601"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8]]},"references-count":43,"journal-issue":{"issue":"8","published-print":{"date-parts":[[1994,8]]}},"alternative-id":["10.1177\/00220345940730080601"],"URL":"https:\/\/doi.org\/10.1177\/00220345940730080601","relation":{},"ISSN":["0022-0345","1544-0591"],"issn-type":[{"value":"0022-0345","type":"print"},{"value":"1544-0591","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,8]]}}}