{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T21:01:54Z","timestamp":1788555714466,"version":"build-2803163510"},"reference-count":22,"publisher":"SAGE Publications","issue":"1","license":[{"start":{"date-parts":[[1997,1,1]],"date-time":"1997-01-01T00:00:00Z","timestamp":852076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[1997,1]]},"abstract":"<jats:p>The role of proteolytic enzymes in the root caries process remains unclear. The aim of this study was to investigate collagenase activity during tooth root demineralization and remineralization in an in vitro demineralization\/remineralization pH-cycling model. Human tooth roots were subjected to pH cycling (alternating demineralization and remineralization) in one of two different time cycles for five days. Collagenase at 90, 180, or 360 \u03bcg per root was placed into either the demineralizing solution or the remineralizing solution in the pH-cycling system. The effects of additional exposure to collagenase before or after pH cycling were also studied. After the exposure, thin sections of the roots were examined histologically by polarized light microscopy. Changes of calcium and phosphate in the solutions were analyzed chemically. Surface erosion occurred only in the groups where collagenase was contained in the remineralizing solution and in which the root samples were exposed to severe demineralization. However, no differences among the control and experimental groups were found in calcium and phosphate changes in the pH-cycling solutions. These findings suggest that collagenase works during the remineralizing phase and predominantly attacks the organic matrix of the root after demineralization. Additional exposure to collagenase before or after pH cycling did not increase surface erosion except for exposure to collagenase in the absence of phosphate following pH cycling.<\/jats:p>","DOI":"10.1177\/00220345970760011001","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"588-595","source":"Crossref","is-referenced-by-count":49,"title":["Effects of Collagenase on Root Demineralization"],"prefix":"10.1177","volume":"76","author":[{"given":"K.","family":"Kawasaki","sequence":"first","affiliation":[{"name":"Department of Preventive Dentistry, Nagasaki University School of Dentistry, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.D.B.","family":"Featherstone","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, University of California San Francisco, 707 Parnassus Ave, San Francisco, California 94143-0758"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"179","published-online":{"date-parts":[[1997,1,1]]},"reference":[{"key":"atypb1","first-page":"20","volume":"1","author":"Billings RJ","year":"1985","journal-title":"Gerodontics"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1990.tb00439.x"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.51.3.765-770.1986"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1986.tb00651.x"},{"key":"atypb5","unstructured":"Clarkson BH, Heilman JR, Hall D., Wefel JS (1987). Effect of proteolytic enzyme and fluoride on in vitro caries like lesion formation in roots of teeth. In: Dentine and dentine reactions in the oral cavity. Thylstrup A, Leach SA, Quist V, editors. Oxford: IRL Press Ltd, pp. 147-154."},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1177\/00220345850640101301"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1177\/00220345850640121001"},{"key":"atypb8","first-page":"271","volume":"7","author":"Featherstone JD","year":"1994","journal-title":"Am J Dent"},{"key":"atypb9","unstructured":"Featherstone JD, Zero DT ( 1992). Laboratory and human studies to elucidate the mechanism of action of fluoride-containing dentifrices. In: Clinical and biological aspects of dentifrices. Embery G, R\u00f8lla G, editors. Oxford: Oxford University Press, pp. 41-50."},{"key":"atypb10","unstructured":"Featherstone JD, O'Reilly MM, Shariati M., Brugler S. (1986). Enhancement of remineralization in vitro and in vivo. In: Factors relating to the demineralization and remineralization of the teeth. Leach S, editor. Oxford, UK: IRL Press, pp. 23-34."},{"key":"atypb11","unstructured":"Featherstone JD, McIntyre JM, Fu J. (1987). Physico-chemical aspects of root caries progression . In: Dentine and dentine reactions in the oral cavity. Thylstrup A, Leach SA , Quist V, editors. Oxford: IRL Press Ltd., pp. 127-137."},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1177\/00220345900690S121"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1159\/000261180"},{"key":"atypb14","first-page":"119","volume":"95","author":"Fure S.","year":"1987","journal-title":"Scand J Dent Res"},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(87)90085-9"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1159\/000261021"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1177\/00220345900690050101"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.58.6.1628-1633.1990"},{"key":"atypb19","doi-asserted-by":"publisher","DOI":"10.1016\/0889-5406(87)90293-9"},{"key":"atypb20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1989.tb00753.x"},{"key":"atypb21","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.11.4.727-731.1975"},{"key":"atypb22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1985.tb00538.x"}],"container-title":["Journal of Dental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00220345970760011001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00220345970760011001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:05:30Z","timestamp":1777496730000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00220345970760011001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,1]]}},"alternative-id":["10.1177\/00220345970760011001"],"URL":"https:\/\/doi.org\/10.1177\/00220345970760011001","relation":{},"ISSN":["0022-0345","1544-0591"],"issn-type":[{"value":"0022-0345","type":"print"},{"value":"1544-0591","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1]]}}}