{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:15:21Z","timestamp":1777810521949,"version":"3.51.4"},"reference-count":41,"publisher":"SAGE Publications","issue":"9","license":[{"start":{"date-parts":[[1997,9,1]],"date-time":"1997-09-01T00:00:00Z","timestamp":873072000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>The extracellular antigens of Actinobacillus actinomycetemcomitans Y4 (serotype b) contain a 37-kDa protein which is a major target for IgGs from patients suffering from severe alveolar bone loss. Since the 37-kDa protein has not been studied sufficiently, our investigation focused on its characteristics, e.g., its localization, specificity, and whether it directly stimulates macrophages to produce cytokines. The 37-kDa protein was purified from the culture supernatant of the Y4 strain by means of chromatofocusing and gel filtration. The 37-kDa protein is a unique glycoprotein which forms immune complexes with monoclonal antibodies against rhamnose-fucose polysaccharide. Patients with A. actinomycetemcomitans-associated periodontitis had higher antibody titers to the purified 37-kDa protein than healthy subjects (p &lt; 0.001). Anti-37-kDa protein antibodies recognized a 37-kDa band in the cytosolic, ribosomal, and total membrane fractions from Y4 cells. Extracellular substances from other strains of A. actinomycetemcomitans (serotypes a and c) also reacted in the Western blots, but Haemophilus spp. or several periodontopathic bacteria did not. These results suggested that the 37-kDa protein is a cytosolic protein that is passed through the cell membrane, and its protein portion is specific for A. actinomycetemcomitans but common to serotypes. This protein induced IL-1\u03b2, IL-6, and TNF-a release from murine macrophages. The IL-6-inducing activity of the 37-kDa protein was higher than that of LPS. These findings suggested that the 37-kDa protein which is released from live cells plays a role in A. actinomycetemcomitans- associated periodontitis, as antigen inducing the release of inflammatory cytokines which are associated with alveolar bone loss.<\/jats:p>","DOI":"10.1177\/00220345970760090501","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"1538-1547","source":"Crossref","is-referenced-by-count":11,"title":["Extracellular 37-kDa Antigenic Protein from Actinobacillus actinomycetemcomitans Induces TNF-\u03b1, IL-1\u03b2, and IL-6 in Murine Macrophages"],"prefix":"10.1177","volume":"76","author":[{"given":"Y.","family":"Tani","sequence":"first","affiliation":[{"name":"Department of Periodontology, Nagasaki University School of Dentistry, 1-7-1 Sakamoto Nagasaki 852, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Tani","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Nagasaki University School of Dentistry, 1-7-1 Sakamoto Nagasaki 852, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Kato","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Nagasaki University School of Dentistry, 1-7-1 Sakamoto Nagasaki 852, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1997,9,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345950740040501"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.57.10.2942-2946.1989"},{"key":"atypb3","doi-asserted-by":"publisher","DOI":"10.1139\/m83-238"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.24.1.233-243.1979"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1990.tb00414.x"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.57.5.1582-1589.1989"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(66)08007-8"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1021\/ac60111a017"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.1177\/10454411900010040601"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.59.5.1795-1802.1991"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.59.6.1972-1977.1991"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V74.1.1.1"},{"key":"atypb13","unstructured":"Koga T., Nishihara T., Amano K., Takahashi T., Nakajima K., Ishihara Y., et al. (1991). Chemical and biological properties of cell surface components of Actinobacillus actinomycetemcomitans. In: Periodontal disease, pathogens & host immune response. Hamada S, Holt SC, McGhee JR, editors. Tokyo: Quintessence, pp. 117-127."},{"key":"atypb14","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1991.tb04326.x"},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(89)90851-6"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1977.tb00107.x"},{"key":"atypb19","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.6.2459-2469.1994"},{"key":"atypb20","unstructured":"Mundy GR, Roodman GD (1987). Osteoclast ontogeny and function. In: Bone and mineral research. Vol. 5. Peck WA, editor. Amsterdam: Elsevier, pp. 209-279."},{"key":"atypb21","doi-asserted-by":"publisher","DOI":"10.2329\/perio.32.71"},{"key":"atypb22","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.56.3.619-625.1988"},{"key":"atypb23","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.2.390-397.1994"},{"key":"atypb24","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.37.1.151-154.1982"},{"key":"atypb25","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(80)90470-4"},{"key":"atypb26","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(85)90273-6"},{"key":"atypb27","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)41496-8"},{"key":"atypb28","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.59.10.3451-3462.1991"},{"key":"atypb29","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650040116"},{"key":"atypb30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1996.tb00473.x"},{"key":"atypb31","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.15.4.606-609.1982"},{"key":"atypb32","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.4.1346-1351.1993"},{"key":"atypb33","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.59.1.18-23.1991"},{"key":"atypb34","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.1995.tb02199.x"},{"key":"atypb35","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1983.tb00375.x"},{"key":"atypb36","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.49.3.487-493.1985"},{"key":"atypb37","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.9.4350"},{"key":"atypb38","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(89)90020-4"},{"key":"atypb39","first-page":"83","volume":"5","author":"Westphal 0","year":"1965","journal-title":"Meth Carbohydr Chem"},{"key":"atypb40","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1991.62.3.211"},{"key":"atypb41","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(72)90433-2"}],"container-title":["Journal of Dental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00220345970760090501","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00220345970760090501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:00:14Z","timestamp":1777496414000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00220345970760090501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,9]]},"references-count":41,"journal-issue":{"issue":"9","published-print":{"date-parts":[[1997,9]]}},"alternative-id":["10.1177\/00220345970760090501"],"URL":"https:\/\/doi.org\/10.1177\/00220345970760090501","relation":{},"ISSN":["0022-0345","1544-0591"],"issn-type":[{"value":"0022-0345","type":"print"},{"value":"1544-0591","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,9]]}}}