{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:41:01Z","timestamp":1777812061340,"version":"3.51.4"},"reference-count":53,"publisher":"SAGE Publications","issue":"10","license":[{"start":{"date-parts":[[1997,10,1]],"date-time":"1997-10-01T00:00:00Z","timestamp":875664000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[1997,10]]},"abstract":"<jats:p>It has recently been shown that mice deficient in activin-\u03b2A subunits and follistatin exhibit major defects in dentition. To increase understanding of the roles played by these molecules during tooth development, we determined the temporospatial expression of activin-\u03b2A subunit and follistatin messenger RNA and their corresponding proteins in developing murine molars (between day E 14 and 2 days after birth). The effects of recombinant human activin A and its binding protein follistatin on odontoblast differentiation were also studied in cultures of dental papillae (DP) isolated from the mandibular first molars of E-17-day mice. In situ hybridization indicated that transcripts for activin-\u03b2A subunit were abundant in pre-odontoblasts at the tips of forming cusps prior to odontoblast terminal differentiation, and transcripts for follistatin in overlying inner enamel epithelial cells (pre-ameloblasts). Pre-odontoblasts were also weakly immunoreactive in relation to activin-\u03b2A subunit, pre-ameloblasts in relation to follistatin. When follistatin was added at different concentrations to a DP culture model (2-14 nmol\/DP) together with heparin at constant concentration, differentiation of odontoblast-like cells was induced, as evidenced by polarization and deposition of extracellular matrix in vitro, to extents depending on the follistatin concentration. In contrast, the addition of activin A (2 nmol\/DP) had no effect on the differentiation parameters studied. These findings suggest that the activin-follistatin system regulates odontoblast differentiation during tooth development. In particular, we suggest that binding of endogenous activin A by follistatin may allow odontoblast terminal differentiation to occur.<\/jats:p>","DOI":"10.1177\/00220345970760100301","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"1625-1636","source":"Crossref","is-referenced-by-count":13,"title":["The Activin-binding Protein Follistatin is Expressed in Developing Murine Molar and Induces Odontoblast-like Cell Differentiation in vitro"],"prefix":"10.1177","volume":"76","author":[{"given":"K.","family":"Heikinheimo","sequence":"first","affiliation":[{"name":"Institut de Biologie Medicale, Universit\u00e9 Louis Pasteur, Faculte de Medecine, INSERM U 424, Strasbourg, France, Institute of Dentistry, University of Turku, FIN-20520 Turku, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Begue-Kirn","sequence":"additional","affiliation":[{"name":"Institut de Biologie Medicale, Universit\u00e9 Louis Pasteur, Faculte de Medecine, INSERM U 424, Strasbourg, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Ritvos","sequence":"additional","affiliation":[{"name":"University of Helsinki, Haartman Institute, Department of Bacteriology and Immunology, FIN-00014 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Tuuri","sequence":"additional","affiliation":[{"name":"University of Helsinki, Haartman Institute, Department of Bacteriology and Immunology, FIN-00014 Helsinki, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.V.","family":"Ruch","sequence":"additional","affiliation":[{"name":"Institut de Biologie Medicale, Universit\u00e9 Louis Pasteur, Faculte de Medecine, INSERM U 424, Strasbourg, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1997,10,1]]},"reference":[{"key":"atypb1","doi-asserted-by":"crossref","first-page":"711","DOI":"10.1242\/dev.117.2.711","volume":"117","author":"Albano RM","year":"1993","journal-title":"Development"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.3.1066"},{"key":"atypb3","first-page":"491","volume":"36","author":"Begue-Kirn C.","year":"1992","journal-title":"Int J Dev Biol"},{"key":"atypb4","first-page":"405","volume":"38","author":"Begue-Kirn C.","year":"1994","journal-title":"Int j Dev Biol"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1994.1069"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650061216"},{"key":"atypb7","doi-asserted-by":"crossref","unstructured":"Decimo D., Georges-Labouesse E., Dolle P. 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