{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T12:19:23Z","timestamp":1777810763800,"version":"3.51.4"},"reference-count":41,"publisher":"SAGE Publications","issue":"7","license":[{"start":{"date-parts":[[1998,7,1]],"date-time":"1998-07-01T00:00:00Z","timestamp":899251200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.sagepub.com\/page\/policies\/text-and-data-mining-license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Dent Res"],"published-print":{"date-parts":[[1998,7]]},"abstract":"<jats:p>Tissue morphogenesis, development, and maintenance of function are mediated by signals generated through the composition of the extracellular matrix. The regulation of the composition of matrix is determined by enzymes specific for their degradation, the matrix metalloproteinases. Chronic injections of the \u03b2-adrenergic receptor agonist, isoproterenol, result in a non-neoplastic hypertrophy and hyperplasia of the rat parotid gland. The activity of matrix metalloproteinases, as measured by gelatin zymography and enzymatic digestion of Azocoll\u00ae substrates by gland lysates, decreased significantly (P &lt; 0.05) following 24 hrs of agonist treatment, and slowly recovered to control values by 6 days of treatment. Daily administration of the broad-spectrum matrix metalloproteinase inhibitor Galardin\u00ae for 3 days in combination with isoproterenol resulted in enhanced gland hypertrophy compared with that produced by isoproterenol alone. Given alone, Galardin\u00ae also caused hypertrophy. The relative abundance of mRNA for the extracellular matrix molecules, collagens I and III and fibronectin, declined rapidly following the initiation of \u03b2-agonist treatment in vivo, while laminin B1 and B2 mRNA levels increased initially before declining below control levels. These changes in patterns of mRNA levels also were observed in the concentrations of glandular protein when Western dot blot analysis of collagens I and III and laminin, respectively, was used. The importance of laminin, in vivo, was demonstrated by co-injection of anti-laminin antibody along with isoproterenol, which resulted in the inhibition of \u03b2-agonist-induced parotid gland hypertrophy and hyperplasia. These data suggest that modulation of the ECM is associated with isoproterenol-induced salivary gland hypertrophy and hyperplasia. It is likely that this modulation of the ECM takes place through transcriptional regulation of some ECM genes and regulation of matrix-degrading enzyme activity.<\/jats:p>","DOI":"10.1177\/00220345980770070501","type":"journal-article","created":{"date-parts":[[2009,1,8]],"date-time":"2009-01-08T19:39:42Z","timestamp":1231443582000},"page":"1504-1514","source":"Crossref","is-referenced-by-count":16,"title":["Changes in the Expression of Extracellular Matrix (ECM) and Matrix Metalloproteinases (MMP) of Proliferating Rat Parotid Acinar Cells"],"prefix":"10.1177","volume":"77","author":[{"given":"R.L.","family":"Broverman","sequence":"first","affiliation":[{"name":"Department of Oral Biology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.-H.T.","family":"Nguyen","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"da Silveira","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610, Department of Orthodontics, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.L.","family":"Brinkley","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610, Department of Orthodontics, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.P.","family":"Macauley","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.W.","family":"Tarnuzzer","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.S.","family":"Schultz","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Kerr","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.G.","family":"Humphreys-Beher","sequence":"additional","affiliation":[{"name":"Department of Oral Biology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610, Department of Pharmacology, P.O. Box 100424, University of Florida Health Science Center, Gainesville, Florida 32610"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[1998,7,1]]},"reference":[{"key":"atypb1","first-page":"4","volume":"68","author":"Albelda SM","year":"1993","journal-title":"Lab Invest"},{"key":"atypb2","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(82)90378-5"},{"key":"atypb3","doi-asserted-by":"crossref","unstructured":"Bernfield MR, Banerjee SD, Koda J., Raprager AC (1984). Remodeling of the basement membrane: morphogenesis and maturation. In: Basement membranes and cell movement. Porter R, Whelan J, editors. Bath, UK: Pittman Press, pp. 179-192.","DOI":"10.1002\/9780470720899.ch12"},{"key":"atypb4","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-775170-2.50009-3"},{"key":"atypb5","doi-asserted-by":"publisher","DOI":"10.1177\/10454411930040020401"},{"key":"atypb6","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"atypb7","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(74)90444-6"},{"key":"atypb8","doi-asserted-by":"publisher","DOI":"10.1038\/1911076a0"},{"key":"atypb9","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-211-43969"},{"key":"atypb10","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(84)90242-2"},{"key":"atypb11","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"atypb12","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.73.2.464"},{"key":"atypb13","doi-asserted-by":"publisher","DOI":"10.1177\/08959374900040010401"},{"key":"atypb14","unstructured":"Cutler LS (1993). Regulation of salivary gland development. In: Biology of salivary glands. Dobrosielski-Vergona K, editor. Boca Raton, FL: CRC Press, pp. 343-353."},{"key":"atypb15","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.102.1.160"},{"key":"atypb16","doi-asserted-by":"publisher","DOI":"10.1021\/bi00146a017"},{"key":"atypb17","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092340315"},{"key":"atypb18","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)89580-1"},{"key":"atypb19","doi-asserted-by":"crossref","first-page":"2013","DOI":"10.1242\/jcs.109.8.2013","volume":"109","author":"Hoffman MP","year":"1996","journal-title":"J Cell Sci"},{"key":"atypb20","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)60868-3"},{"key":"atypb21","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-193-43038"},{"key":"atypb22","doi-asserted-by":"crossref","unstructured":"Hunt S. ( 1981). Collagen and embryonic development. In: Cell biology of extracellular matrix. Hay ED, editor. New York, NY: Plenum Press, pp. 379-405.","DOI":"10.1007\/978-1-4613-0881-2_13"},{"key":"atypb23","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90233-9"},{"key":"atypb24","first-page":"261","volume":"907","author":"Juliano RL","year":"1987","journal-title":"Biochim Biophys Acta"},{"key":"atypb25","unstructured":"Lazowski KW, Mertz PM, Redman RS, Kousvelari E. (1994)."},{"key":"atypb26","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90353-2"},{"key":"atypb27","doi-asserted-by":"crossref","unstructured":"MacKay SLD, Bennett NT, Bland KI, Schultz GS (1992). Growth factors, tumor suppressors and cancer. Persp Gen Surg 3:1-25. Maillett CM, Shur BD (1994). Perturbing cell surface \u03b2-(1,4) galactosyltransferase on F9 embryonal carcinoma cells arrests cell growth and induces laminin synthesis. J Cell Sci 108:1713-1724.","DOI":"10.1242\/jcs.107.6.1713"},{"key":"atypb28","first-page":"453","volume":"36","author":"Marchase RB","year":"1988","journal-title":"J Cell Biol"},{"key":"atypb29","first-page":"209","volume":"66","author":"Nagawa H.","year":"1981","journal-title":"J Embryol Exp Morphol"},{"key":"atypb30","unstructured":"Nakanishi Y, Sugiura F, Kishi J, Hayakawa T (1986). Collagenase inhibitor stimulates cleft formation during early morphogenesis of mouse salivary gland. Dev Biol 114:201-206. Purushotham KR, Humphreys-Beher MG (1995). The role of phosphotyrosine signaling pathway in parotid gland proliferation and function. Crit Rev Oral Biol Med 6:119-131. Purushotham KR, Dunn WA Jr, Schneyer CA, Humphreys-Beher MG (1992). A novel mechanism of isoprenaline-stimulated proliferation of rat parotid acinar cells involving the epidermal growth factor receptor and cell surface galactosyltransferase. Biochem J 284:764-776."},{"key":"atypb31","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.107.5.1863"},{"key":"atypb32","doi-asserted-by":"publisher","DOI":"10.1126\/science.2821619"},{"key":"atypb33","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1962.203.2.232"},{"key":"atypb34","doi-asserted-by":"crossref","unstructured":"Selye HR, Veilleux R, Cantin M (1961). Excessive stimulation of salivary gland growth by isoproterenol. Science 133:44-45. Shaw PA, Barka T (1989). Beta-adrenergic induction of a cysteine-protease-inhibitor mRNA in rat salivary glands. Biochem J 257:685-689.","DOI":"10.1126\/science.133.3445.44"},{"key":"atypb35","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)81334-0"},{"key":"atypb36","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/1.6.563"},{"key":"atypb37","doi-asserted-by":"publisher","DOI":"10.1016\/0955-0674(93)90035-O"},{"key":"atypb38","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(11)80398-5"},{"key":"atypb39","doi-asserted-by":"publisher","DOI":"10.1021\/bi00496a016"},{"key":"atypb40","doi-asserted-by":"crossref","unstructured":"Tarnuzzer RW, Macauley SP, Farmerie WG, Caballero S, Ghassemifar MR, Anderson JT, et al. (1996). Competitive RNA templates for detection and quantitation of growth factors, cytokines, extracellular matrix components and matrix metalloproteinases by RT-PCR. Biotechniques 20:670-674. Thompson HA, Spooner BS (1983). Proteoglycan and glycosaminoglycan synthesis in embryonic mouse salivary glands: effects of beta-D-xyloside, an inhibitor of branching of morphogenesis. J Cell Biol 96:1443-1550.","DOI":"10.1083\/jcb.96.5.1443"},{"key":"atypb41","doi-asserted-by":"crossref","unstructured":"Woesner JF Jr (1991). Matrix metalloproteases and their inhibitors in connective tissue remodeling. FASEB J 5:2145-2154. Yamada KM (1988). Fibronectin domains and receptors. In: Fibronectin. Mosher DF, editor. New York, NY: Academic Press Inc., pp. 47-121.","DOI":"10.1096\/fasebj.5.8.1850705"}],"container-title":["Journal of Dental Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00220345980770070501","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.sagepub.com\/doi\/pdf\/10.1177\/00220345980770070501","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T21:00:49Z","timestamp":1777496449000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.sagepub.com\/doi\/10.1177\/00220345980770070501"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,7]]},"references-count":41,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1998,7]]}},"alternative-id":["10.1177\/00220345980770070501"],"URL":"https:\/\/doi.org\/10.1177\/00220345980770070501","relation":{},"ISSN":["0022-0345","1544-0591"],"issn-type":[{"value":"0022-0345","type":"print"},{"value":"1544-0591","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,7]]}}}