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The objectives of this study were to determine whether COX isoenzymes are expressed in porcine gastric ulcers and to characterize the porcine COX-2 cDNA. Normal stomachs ( n=5) and those with gastric ulcers ( n=35) were studied by immunohistochemistry and immunoblotting analysis. Reverse transcription-polymerase chain reaction (RT-PCR) was used to isolate the complete porcine COX-2 cDNA. COX-1 staining was present in normal stomach and in ulcerated areas. No COX-2 was detected in normal stomach, but COX-2 was strongly expressed in the ulcerated area in 28\/35 (80%) gastric ulcers ( p&lt;0.01). Immunoblotting analysis confirmed the restricted expression of COX-2 in the ulcerated areas. The porcine COX-2 cDNA was shown to code for a 604 amino acid protein that is 89% identical to human COX-2. These results provide the complete primary structure of porcine COX-2 and demonstrate for the first time that the enzyme is induced in naturally occurring porcine gastric ulcers.<\/jats:p>","DOI":"10.1177\/002215540205000706","type":"journal-article","created":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T03:17:54Z","timestamp":1301627874000},"page":"923-933","source":"Crossref","is-referenced-by-count":5,"title":["Induction of Cyclo-oxygenase-2 Expression in Naturally Occurring Gastric Ulcers"],"prefix":"10.1177","volume":"50","author":[{"given":"St\u00e9phane","family":"Lajoie","sequence":"first","affiliation":[{"name":"D\u00e9partement de Pathologie et Microbiologie Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, Qu\u00e9bec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Sirois","sequence":"additional","affiliation":[{"name":"Centre de Recherche en Reproduction Animale, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, Qu\u00e9bec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monique","family":"Dor\u00e9","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Pathologie et Microbiologie Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, Qu\u00e9bec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"179","published-online":{"date-parts":[[2002,7,1]]},"reference":[{"key":"bibr1-002215540205000706","doi-asserted-by":"publisher","DOI":"10.1097\/00004836-199800001-00003"},{"key":"bibr2-002215540205000706","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8436"},{"key":"bibr3-002215540205000706","doi-asserted-by":"publisher","DOI":"10.1177\/030098589503200206"},{"key":"bibr4-002215540205000706","doi-asserted-by":"publisher","DOI":"10.1210\/endo.139.4.5898"},{"key":"bibr5-002215540205000706","doi-asserted-by":"publisher","DOI":"10.1210\/endo.138.1.4889"},{"key":"bibr6-002215540205000706","first-page":"751","volume":"51","author":"Brzozowski T","year":"2000","journal-title":"J Physiol Pharmacol"},{"key":"bibr7-002215540205000706","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.83.6.1670"},{"key":"bibr8-002215540205000706","first-page":"27","volume-title":"Methods in Enzymology","author":"Catafalmo J","year":"1989"},{"issue":"161","key":"bibr9-002215540205000706","first-page":"164","volume":"64","author":"C\u00f4t\u00e9 F","year":"2001","journal-title":"Biol Reprod"},{"key":"bibr10-002215540205000706","unstructured":"Cotran R, Kumar V, Collins T, eds. 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